TAILIEUCHUNG - Báo cáo khoa học: The shell matrix of the freshwater mussel Unio pictorum (Paleoheterodonta, Unionoida) Involvement of acidic polysaccharides from glycoproteins in nacre mineralization

Among molluscs, the shell biomineralization process is controlled by a set of extracellular macromolecular components secreted by the calcifying mantle. In spite of several studies, these components are mainly known in bivalves from only few members of pteriomorph groups. | ỊFEBS Journal The shell matrix of the freshwater mussel Unio pictorum Paleoheterodonta Unionoida Involvement of acidic polysaccharides from glycoproteins in nacre mineralization Benjamin Marie1 Gilles Luquet1 Jean-Paul Pais De Barros2 Nathalie Guichard1 Sylvain Morel3 Gerard Alcaraz3 Loic Bollache1 and Frederic Marin1 1 UMR CNRS 5561 Biogeosciences Universite de Bourgogne Dijon France 2 U 498 INSERM Metabolisme des lipoproteines humaines et interactions vasculaires Universite de Bourgogne Dijon France 3 UMR INRA692 Biochimie des interactions cellulaires ENESAD Dijon France Keywords biomineralization calcium-binding protein glycoprotein matrix macromolecules mollusc shell nacre Correspondence B. Marie G. Luquet or F. Marin UMR 5561 CNRS Biogeosciences Universite de Bourgogne 6 boulevard Gabriel F-21000 France Fax 33 3 80 39 63 87 Tel 33 3 80 39 63 72 E-mail Received 27 December 2006 revised 13 March 2007 accepted 10 April 2007 doi Among molluscs the shell biomineralization process is controlled by a set of extracellular macromolecular components secreted by the calcifying mantle. In spite of several studies these components are mainly known in bivalves from only few members of pteriomorph groups. In the present case we investigated the biochemical properties of the aragonitic shell of the freshwater bivalve Unio pictorum Paleoheterodonta Unionoida . Analysis of the amino acid composition reveals a high amount of glycine aspartate and alanine in the acid-soluble extract whereas the acid-insoluble one is rich in alanine and glycine. Monosaccharidic analysis indicates that the insoluble matrix comprises a high amount of glucosamine. Furthermore a high ratio of the carbohydrates of the soluble matrix is sulfated. Electrophoretic analysis of the acid-soluble matrix revealed discrete bands. Stains-All Alcian Blue periodic acid Schiff and .

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