TAILIEUCHUNG - Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer

The AAA peroxins, Pex1p and Pex6p, are components of the peroxisomal protein import machinery required for the relocation of the import receptor Pex5p from the peroxisomal membrane to the cytosol. We demonstrate that Pex1p and Pex6p form a stable complex in the cytosol, which associ-ates at the peroxisomal membrane with their membrane anchor Pex15p and the peroxisomal importomer. | ễFEBS Journal Functional association of the AAA complex and the peroxisomal importomer Katja Rosenkranz Ingvild Birschmann t Silke Grunau Wolfgang Girzalsky Wolf-H. Kunau and Ralf Erdmann Abteilung fur Systembiochemie Medizinische Fakultat der Ruhr-Universitat Bochum Germany Keywords AAA ATPases associated with various cellular activities proteins peroxisome biogenesis Pex1p Pex6p Pex15p Correspondence R. Erdmann Institut fur Physiologische Chemie Abteilung fur Systembiochemie Medizinische Fakultat der Ruhr-Universitat Bochum D-44780 Bochum Germany Fax 49 234321 4266 Tel 49 234322 4943 E-mail These authors contributed equally to this paper tPresent address Institut fijr Klinische Biochemie und Pathobiochemie Medizinische Universitatsklinik D-97078 Wurzburg Germany The AAA peroxins Pexlp and Pex6p are components of the peroxisomal protein import machinery required for the relocation of the import receptor Pex5p from the peroxisomal membrane to the cytosol. We demonstrate that Pexlp and Pex6p form a stable complex in the cytosol which associates at the peroxisomal membrane with their membrane anchor Pexl5p and the peroxisomal importomer. The interconnection of Pexl5p with the components of the importomer was independent of Pexlp and Pex6p indicating that Pexl5p is an incorporated component of the assembly. Further evidence suggests that the AAA peroxins shuttle between cytosol and peroxisome with proper binding of the Pex15p-AAA complex to the impor-tomer and release of the AAA peroxins from the peroxisomal membrane depending on an operative peroxisomal protein import mechanism. Pex4p-deficient cells exhibit a wild-type-like assembly of the importomer which differs in that it is associated with increased amounts of Pex1p and Pex6p in agreement with a function for Pex4p in the release of AAA peroxins from the peroxisomal membrane. Received 26 April2006 revised 13 June 2006 accepted 20 June 2006 doi Peroxisomes .

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