TAILIEUCHUNG - Báo cáo khoa học: Molecular mass of macromolecules and subunits and the quaternary structure of hemoglobin from the microcrustacean Daphnia magna

The molecular masses of macromolecules and subunits of the extracellular hemoglobin from the fresh-water crustaceanDaphnia magnawere deter-mined by analytical ultracentrifugation, multiangle laser light scattering and electrospray ionization mass spectrometry. | ềFEBS Journal Molecular mass of macromolecules and subunits and the quaternary structure of hemoglobin from the microcrustacean Daphnia magna T Pt Ia Q I a m Ixzt m Q Q r1 2 R otti n o 7QỈQ1 Noin7 rior I ot 3 c I tri a f laoniol Q3 XA iscr kikii icnh3 lUUlao Lai I Ikei I leyei Dcttllla Zeis Heinz DcUkcl Limdl JauHIUku Dieiei VvaoUMUUoUH XA rtlfnonn Cm a Pt u I I a r4 11 I a n l larlzl4 I llrirh l lQÌQQnQr4 MnrHanQ RAiiccQlAt 7ul5 vvoiiyany Geuauei uuiyeH Iviaiki Uiiich ivieiooHei Ivioiyane nuuooeiui iiaHUk zai Graeme J. Nicholson6 and Rudiyer J. Paul1 1 Institut fur Zoophysiologie Westfalische Wilhelms-Universitat Munster Germany 2 Proteom Centrum Tubingen Eberhard-Karls-Universitat Tubingen Germany 3 Institut fur Molekulare Biophysik Johannes Gutenberg Universitat Mainz Germany 4 Institut fur Zoologie Johannes Gutenberg Universitat Mainz Germany 5 Equipe Ecophysiologie Adaptation et Evolution Moleculaires UPMC-CNRS UMR 7144 Station Biologique BP 74 Roscoff France 6 Institut fuur Organische Chemie Eberhard-Karls-Universitat Tubingen Germany Keywords glycosylation hemoglobin macromolecule molecular mass quaternary structure Correspondence T. Lamkemeyer Interfakultares Institut fuur Zellbiologie Proteom Centrum Tubingen Eberhard-Karls-Universitat Auf der Morgenstelle 15 D-72076 Tubingen Germany Fax 7071 295359 Tel 7071 2970556 E-mail Received 23 March 2006 revised 17 May 2006 accepted 30 May 2006 doi The molecular masses of macromolecules and subunits of the extracellular hemoglobin from the fresh-water crustacean Daphnia magna were determined by analytical ultracentrifugation multiangle laser light scattering and electrospray ionization mass spectrometry. The hemoglobins from hypoxia-incubated hemoglobin-rich and normoxia-incubated hemoglobinpoor Daphnia magna were analyzed separately. The sedimentation coefficient of the macromolecule was S and its molecular mass was 583 kDa .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• oxidation
• Crystal structure
• bacteria
• medical knowledge
• medical research
• analysis of cytoplasmic
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine