TAILIEUCHUNG - Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding

In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. | iFEBS Journal CpG methylation of the CENP-B box reduces human CENP-B binding Yoshinori Tanaka1 2 Hitoshi Kurumizaka1 3 and Shigeyuki Yokoyama1 2 4 1 Protein Research Group RIKEN Genomic Sciences Center Suehiro-cho Tsurumi Yokohama Japan 2 Department of Biophysics and Biochemistry Graduate Schoolof Science University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo Japan 3 Waseda University Schoolof Science and Engineering 3-4-1 Okubo Shinjuku-ku Tokyo Japan 4 RIKEN Harima Institute at Spring-8 1-1-1 Kohto Mikazuki-cho Sayo Hyogo Japan Keywords CENP-B centromere DNA methylation chromatin heterochromatin Correspondence H. Kurumizaka Waseda University Schoolof Science and Engineering 3-4-1 Okubo Shinjuku-ku Tokyo 169-8555 Japan Fax 81 3 5292 9211 Tel 81 3 5286 8189 E-mail kurumizaka@ S. Yokoyama Protein Research Group RIKEN Genomic Sciences Center 1-7-22 Suehiro-cho Tsurumi Yokohama 230-0045 Japan Fax 81 45 503 9195 Tel 81 45 503 9196 E-mail yokoyama@ In eukaryotes CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B CENP-B specifically binds to the centromeric 17 base-pair CENP-B box DNA which contains two CpG dinucleotides. In this study we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation. Present address Toray Industries Inc. New Frontiers Research Laboratories 1111 Tebiro Kamakura Kanagawa 248-0036 Japan Received 15 September 2004 accepted 1 October 2004 doi The centromere of eukaryotic chromosomes plays an essential role in the proper segregation of chromosomes at mitosis and meiosis and has a special heterochromatin structure which is composed of .

TÀI LIỆU LIÊN QUAN
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.