TAILIEUCHUNG - Báo cáo khoa học: On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans

Propionate is used to protect bread and animal feed from moulds. The mode of action of this short-chain fatty acid was studied usingAspergillus nidulansas a model organism. The filamentous fungus is able to grow slowly on propio-nate, which is oxidized to acetyl-CoA via propionyl-CoA, methylcitrate and pyruvate. Propionate inhibits growth of A. nidulanson glucose but not on acetate; the latter was shown to inhibit propionate oxidation. | Eur. J. Biochem. 271 3227-3241 2004 FEBS 2004 doi On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans Matthias Brock1 and Wolfgang Buckel2 1 Laboratorium fur Mikrobiologie Universitat Hannover 2Laboratorium fur Mikrobiologie Fachbereich Biologie Philipps-Universităt Marburg Germany Propionate is used to protect bread and animal feed from moulds. The mode of action of this short-chain fatty acid was studied using Aspergillus nidulans as a model organism. The filamentous fungus is able to grow slowly on propionate which is oxidized to acetyl-CoA via propionyl-CoA methylcitrate and pyruvate. Propionate inhibits growth of A. nidulans on glucose but not on acetate the latter was shown to inhibit propionate oxidation. When grown on glucose a methylcitrate synthase deletion mutant is much more sensitive towards the presence of propionate in the medium as compared to the wild-type and accumulates 10-fold higher levels of propionyl-CoA which inhibits CoA-dependent enzymes such as pyruvate dehydrogenase succi-nyl-CoA synthetase and ATP citrate lyase. The most important inhibition is that of pyruvate dehydrogenase as this affects glucose and propionate metabolism directly. In contrast the blocked succinyl-CoA synthetase can be circumvented by a succinyl-CoA acetate propionate CoA-transferase whereas ATP citrate lyase is required only for biosynthetic purposes. In addition data are presented that correlate inhibition of fungal polyketide synthesis by pro-pionyl-CoA with the accumulation of this CoA-derivative. A possible toxicity of propionyl-CoA for humans in diseases such as propionic acidaemia and methylmalonic aciduria is also discussed. Keywords acetate CoA-transferase succinyl-CoA poly-ketide synthesis pyruvate dehydrogenase pyruvate excretion. Sodium propionate is widely used as a preservative due to its ability to inhibit fungal growth. Furthermore this .

TÀI LIỆU LIÊN QUAN
TÀI LIỆU MỚI ĐĂNG
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.