TAILIEUCHUNG - Báo cáo khoa học: Chloroplast phosphoglycerate kinase from Euglena gracilis Endosymbiotic gene replacement going against the tide

Two chloroplast phosphoglycerate kinase isoforms fromthe photosynthetic flagellateEuglena graciliswere purified to homogeneity, partially sequenced, and subsequently cDNAs encoding phosphoglycerate kinase isoenzymes from both the chloroplast and cytosol of E. graciliswere cloned and sequenced. Chloroplast phosphoglycerate kinase, a mono-meric enzyme, was encoded as a polyprotein precursor of at least four mature subunits that were separated by conserved tetrapeptides. | Eur. J. Biochem. 271 4123-4131 2004 FEBS 2004 doi Chloroplast phosphoglycerate kinase from Euglena gracilis Endosymbiotic gene replacement going against the tide Ulrich Nowitzki1 Gabriel Gelius-Dietrich1 Maike Schwieger2 Katrin Henze1 and William Martin1 1 Institute of Botany III Heinrich-Heine-University Dusseldorf Germany 2Heinrich-Pette-Institute for Experimental Virology and Immunology at the University of Hamburg Germany Two chloroplast phosphoglycerate kinase isoforms from the photosynthetic flagellate Euglena gracilis were purified to homogeneity partially sequenced and subsequently cDNAs encoding phosphoglycerate kinase isoenzymes from both the chloroplast and cytosol of E. gracilis were cloned and sequenced. Chloroplast phosphoglycerate kinase a monomeric enzyme was encoded as a polyprotein precursor of at least four mature subunits that were separated by conserved tetrapeptides. In a Neighbor-Net analysis of sequence similarity with homologues from numerous prokaryotes and eukaryotes cytosolic phosphoglycerate kinase of E. gracilis showed the highest similarity to cytosolic and glycosomal homologues from the Kinetoplastida. The chloroplast isoenzyme of E. gracilis did not show a close relationship to sequences from other photosynthetic organisms but was most closely related to cytosolic homologues from animals and fungi. Keywords endosymbiotic gene replacement Euglena gracilis phosphoglycerate kinase polyproteins. The complex chloroplasts of the photosynthetic flagellate Euglena gracilis are surrounded by three membranes evidence for their origin through secondary endosymbiosis 1 . The two partners involved in this endosymbiotic event are thought to be a relative of extant Kinetoplastida as host cell and a green alga as endosymbiont. Euglena gracilis is linked to the Kinetoplastida by a number of morphological homologies 2-7 and shares unique characters such as the kinetoplastid-specific redox enzyme trypanothione .

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