TAILIEUCHUNG - Báo cáo khoa học: Three novel carp CXC chemokines are expressed early in ontogeny and at nonimmune sites

Three novel CXC chemokines were identified in common carp (Cyprinus carpioL.) through homology cloning. Phy-logenetic analyses show that one of the three CXC chemo-kines is an unambiguous orthologue ofCXCL14,whereas both others are orthologues ofCXCL12,and were named CXCL12aandCXCL12b. Percentages of amino acid iden-tity between each of these carp chemokines and their human and mouse orthologues are markedly higher than those reported previously for other carp CXC chemokines, sug-gestive of involvement invital processes, whichhave allowed for relatively few structural changes. . | Eur. J. Biochem. 271 4094-4106 2004 FEBS 2004 doi Three novel carp CXC chemokines are expressed early in ontogeny and at nonimmune sites Mark O. Huising1 2 Talitha van der Meulen3 Gert Flik2 and B. M. Lidy Verburg-van Kemenade1 1 Department of Cell Biology and Immunology Wageningen University the Netherlands 2 Department of Animal Physiology Radboud University Nijmegen the Netherlands 3Department of Experimental Zoology Wageningen University the Netherlands Three novel CXC chemokines were identified in common carp Cyprinus carpio L. through homology cloning. Phylogenetic analyses show that one of the three CXC chemo-kines is an unambiguous orthologue of CXCL14 whereas both others are orthologues of CXCL12 and were named CXCL12a and CXCL12b. Percentages of amino acid identity between each of these carp chemokines and their human and mouse orthologues are markedly higher than those reported previously for other carp CXC chemokines suggestive of involvement in vital processes which have allowed for relatively few structural changes. Furthermore all three novel carp CXC chemokines are expressed during early development in contrast to established immune CXC chemokines. In noninfected adult carp CXCL12b and CXCL14 are predominantly expressed in the brain. CXCL12a is highly expressed in kidney and anterior kidney but its expression is still more abundant in brain than any other carp CXC chemokine. Clearly these chemokines must play key roles in the patterning and maintenance of the developing vertebrate central nervous system. Keywords central nervous system CXC chemokine cXcL12 CXCL14 fish. Chemokines are small proteins that derive their name from their chemotactic properties. Chemokine is an acronym for chemotactic cytokine and reflects their discovery and characterization as important chemoattractants in the pro-inflammatory phase of the immune response. Based on the pattern and spacing of four conserved cysteine residues that determine

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