TAILIEUCHUNG - Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain

SOCS3 is a negative regulator of cytokine signalling that inhibits Janus kinase-signal transduction and activator of transcription (JAK-STAT) mediated signal tranduction by binding to phosphorylated tyrosine residues on intracellular subunits of various cytokine receptors, as well as possibly the JAK proteins. | iFEBS Journal Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain I R ihnn1 Qhonnnon Van1 David p riiaPm 17a 1 r h rictnnhiaf F I I a rricnn 1 I o I lie I Fahri2 Jciiicy u. LJ JUU Oiienggen I du Lvav I m r . ucuuuzd Wiuistopiiei r. I Idiiisui I Luuis u. ra UI I Edvards Liepinsh3 Sergio D. Scrofani2 Manuel Baca1 and Raymond S. Norton1 1 Walter and Eliza Hall institute Parkville Victoria Australia 2 Amrad Corporation Ltd Richmond Victoria Australia 3 Department of MedicalBiochemistry and Biophysics Karolinska Institute Stockholm Sweden Keywords cytokine signalling NMR PEST sequence SOCS Correspondence J. J. Babon Walter and Eliza Hall institute 1G RoyalParade Parkville 3050 Victoria Australia Fax 61 3 93470852 Tel 61 3 93452451 E-mail babon@ Present addresses Schoolof Biochemistry University of Melbourne Parkville 3050 Australia TAmrad Corporation Ltd 576 Swan Street Richmond 3121 Victoria Australia Received 31 July 2005 revised 22 September 2005 accepted 10 October 2005 doi SOCS3 is a negative regulator of cytokine signalling that inhibits Janus kinase-signal transduction and activator of transcription JAK-STAT mediated signal tranduction by binding to phosphorylated tyrosine residues on intracellular subunits of various cytokine receptors as well as possibly the JAK proteins. SOCS3 consists of a short N-terminal sequence followed by a kinase inhibitory region an extended SH2 domain and a C-terminal suppressor of cytokine signalling SOCS box. SOCS3 and the related protein cytokine-inducible SH2-containing protein are unique among the SOCS family of proteins in containing a region of mostly low complexity sequence between the SH2 domain and the C-terminal SOCS box. Using NMR we assigned and determined the secondary structure of a murine SOCS3 construct. The SH2 domain unusually consists of 140 residues including an unstructured .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine