TAILIEUCHUNG - Báo cáo khoa học: The myeloid leukemia factor interacts with COP9 signalosome subunit 3 in Drosophila melanogaster

The humanmyeloid leukemia factor 1(hMLF1) gene was first identified as anNPM–hMLF1fusion gene produced by chromosomal translocation. In Drosophila, dMLF has been identified as a protein homologous to hMLF1 and hMLF2, which interacts with various factors involved in transcrip-tional regulation. | ễFEBS Journal The myeloid leukemia factor interacts with COP9 signalosome subunit 3 in Drosophila melanogaster Wakana Sugano1 2 Katsuhito Ohno1 Noriko Yoneda-Kato3 Jun-ya Kato3 and Masamitsu Yamaguchi1 2 1 Department of Applied Biology Kyoto Institute of Technology Japan 2 Insect BiomedicalResearch Center Kyoto Institute of Technology Japan 3 Graduate Schoolof BiologicalSciences Nara Institute of Science and Technology Japan Keywords COP9 signalosome CSN3 cyclin E dMLF Drosophila Correspondence M. Yamaguchi Department of Applied Biology Kyoto Institute of Technology Matsugasaki Sakyo-ku Kyoto 606-8585 Japan Fax 81 75 724 7760 Tel 81 75 724 7781 E-mail myamaguc@ Present address Department of Molecular Biology and Biochemistry The State University of New Jersey Piscataway NJ USA Received 19 October 2007 revised 5 December 2007 accepted 10 December 2007 doi The human myeloid leukemia factor 1 hMLF1 gene was first identified as an NPM-hMLF1 fusion gene produced by chromosomal translocation. In Drosophila dMLF has been identified as a protein homologous to hMLF1 and hMLF2 which interacts with various factors involved in transcriptional regulation. However the precise cellular function of dMLF remains unclear. To generate further insights we first examined the behavior of dMLF protein using an antibody specific to dMLF. Immunostaining analyses showed that dMLF localizes in the nucleus in early embryos and cultured cells. Ectopic expression of dMLF in the developing eye imaginal disc using eyeless-GAL4 driver resulted in a small-eye phenotype and co-expression of cyclin E rescued the small-eye phenotype suggesting the involvement of dMLF in cell-cycle regulation. We therefore analyzed the molecular mechanism of interactions between dMLF and a dMLF-interact-ing protein dCSN3 a subunit of the COP9 signalosome which regulates multiple signaling and cell-cycle pathways. Biochemical and genetic analyses revealed that dMLF interacts .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• biochemical studies
• environmental medicine
• Crystal structure
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells