TAILIEUCHUNG - Báo cáo hóa học: " Optimizing atomic force microscopy for characterization of diamond-protein interfaces"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Optimizing atomic force microscopy for characterization of diamond-protein interfaces | Rezek et al. Nanoscale Research Letters 2011 6 337 http content 6 1 337 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Optimizing atomic force microscopy for characterization of diamond-protein interfaces Bohuslav Rezek Egor Ukraintsev and Alexander Kromka Abstract Atomic force microscopy AFM in contact mode and tapping mode is employed for high resolution studies of soft organic molecules fetal bovine serum proteins on hard inorganic diamond substrates in solution and air. Various effects in morphology and phase measurements related to the cantilever spring constant amplitude of tip oscillations surface approach tip shape and condition are demonstrated and discussed based on the proposed schematic models. We show that both diamond and proteins can be mechanically modified by Si AFM cantilever. We propose how to choose suitable cantilever type optimize scanning parameters recognize and minimize various artifacts and obtain reliable AFM data both in solution and in air to reveal microscopic characteristics of protein-diamond interfaces. We also suggest that monocrystalline diamond is well defined substrate that can be applicable for fundamental studies of molecules on surfaces in general. Background Diamond is an attractive material for merging solid-state and biological systems 1-7 . Polished monocrystalline diamonds MCDs exhibit low enough surface roughness RMS 1 nm so that they are suitable for morphology and thickness determination of thin proteins layers 8 9 . Diamond is also chemically inert yet its surface can be functionalized by different atoms and even organic molecules 1 4 that lead to diverse functionality of diamond surfaces. The most representative and the most widely employed instances are hydrogen and oxygen surface atoms. Hydrogen-terminated diamond H-dia-mond surface is hydrophobic while oxygen-terminated diamond O-diamond surface is hydrophilic. Those and other properties of diamond-solution .

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