TAILIEUCHUNG - Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies

The characterization of membrane proteins having no identified function in Mycobacterium tuberculosisis important for a better understanding of the biology of this pathogen. In this work, the biological activity of the Rv2560 protein was characterized and evaluated. | ễFEBS Journal The Mycobacterium tuberculosis membrane protein Rv2560 - biochemical and functional studies David F. Plaza1 Hernando Curtidor2 4 Manuel A. Patarroyo1 4 Julie A. Chapeton-Montes1 Claudia Reyes3 Jose Barreto3 and Manuel E. Patarroyo1 5 1 Molecular Biology Department Fundacion Institute de Inmunologia de Colombia Bogota Colombia 2 Receptor-Ligand Department Fundacion Instituto de Inmunologia de Colombia Bogota Colombia 3 ChemicalSynthesis Department Fundacion Instituto de Inmunologia de Colombia Bogota Colombia 4 Universidad delRosario Bogota Colombia 5 Universidad Nacionalde Colombia Bogota Colombia Keywords high-activity binding peptide invasion inhibition Mycobacterium tuberculosis -host cell interaction Rv2560 membrane protein Correspondence M. E. Patarroyo Carrera 50 26-00 Bogota Colombia Fax 57 1 4815269 Tel 57 1 4815219 or 57 1 3244672 Ext 125 E-mail mepatarr@ Received 31 July 2007 revised 11 October 2007 accepted 17 October 2007 doi The characterization of membrane proteins having no identified function in Mycobacterium tuberculosis is important for a better understanding of the biology of this pathogen. In this work the biological activity of the Rv2560 protein was characterized and evaluated. Primers used in PCR and RT-PCR assays revealed that the gene encoding protein Rv2560 is present in M. tuberculosis complex strains but transcribed in only some of them. Sera obtained from rabbits inoculated with polymer peptides from this protein recognized a 33 kDa band in the M. tuberculosis lysate and a membrane fraction corresponding to the predicted molecular mass kDa of this protein. Immunoelectron microscopy analysis found this protein on the mycobacterial membrane. Sixteen peptides covering its entire length were chemically synthesized and tested for their ability to bind to A549 and U937 cells. Peptide 11024 121 VRALSDRATtAyTNTSGVSS140 showed high specific binding to both cell types dissociation .

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