TAILIEUCHUNG - Encyclopedia of Smart Materials (Vols 1 and 2) - M. Schwartz (2002) Episode 10

Tham khảo tài liệu 'encyclopedia of smart materials (vols 1 and 2) - m. schwartz (2002) episode 10', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | temperature results in transition of the surface to the hydrophilic state where the cells can be easily detached from the solid substrate without any damage. Poly NIPAAM was grafted to polystyrene culture dishes using an electron beam. Bovine hepatocytes cells that are highly sensitive to enzymatic treatment were cultivated for 2 days at 37 C and detached by incubation at 4 C for 1 h. Nearly 100 of the hepatocytes was detached and recovered from the poly NIPAAM -grafted dishes by low-temperature treatment whereas only about 8 of the cells was detached from the control dish 57 . The technique has been extended to different cell types 58 59 . It is noteworthy that hepatocytes recovered by cooling retained their native form had numerous bulges and dips and attach well to the hydrophobic surface again for example when the temperature was increased above the conformational transition of poly NIPAAM . On the contrary enzyme-treated cells had a smooth outer surface and had lost their ability to attach to the surface. Thus cells recovered by a temperature shift from poly NIPAAM -grafted surfaces have an intact structure and maintain normal cell functions 58 . The molecular machinery involved in cell-surface detachment was investigated using temperature-responsive surfaces 60 . Poly NIPAAM -grafted and nongrafted surfaces showed no difference in attachment spreading growth confluent cell density or morphology of bovine aortic endothelial cells at 37 C. Stress fibers peripheral bands and focal contacts were established in similar ways. When the temperature was decreased to 20 C the cells grown on poly NIPAAM -grafted support lost their flattened morphology and acquired a rounded appearance similar to that of cells immediately after plating. Mild agitation makes the cells float free from the surface without a trypsin treatment. Neither changes in cell morphology nor cell detachment occurred on ungrafted surfaces. Sodium azide an ATP synthesis inhibitor and genistein a .

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