TAILIEUCHUNG - Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor | Journal of Negative Results in BioMedicine BioMed Central Research Open Access VEGF receptors on PC12 cells mediate transient activation of ERK1 2 and Akt comparison of nerve growth factor and vascular endothelial growth factor Ingrid Berger Sonja Stahl Natalia Rychkova and Ute Felbor Address Department of Human Genetics University of Wurzburg Germany Email Ingrid Berger - ingrid_berger@ Sonja Stahl - Natalia Rychkova - nrych@ Ute Felbor - felbor@ Corresponding author Published 01 June 2006 Received 04 February 2006 Journal of Negative Results in BioMedicine 2006 5 8 doi 1477-5751-5-8 Accepted OIJune 2006 This article is available from http content 5 1 8 2006 Berger et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Vascular endothelial growth factor VEGF and endostatin are angiogenic and anti-angiogenic molecules respectively that have been implicated in neurogenesis and neuronal survival. Using alkaline phosphatase fusion proteins we show that the PC12 neuronal cell line contains cell membrane receptors for VEGF but not for endostatin and the collagen XV endostatin homologue. Immunocytochemistry confirmed that proliferating and differentiated PC12 cells express VEGF receptors 1 2 and neuropilin-1. While no functional effects of VEGF on PC12 cell proliferation and differentiation could be observed a slight VEGF-induced reduction of caspase-3 activity in differentiated apoptotic PC12 cells was paralleled by transient activation of ERK1 2 and Akt. In direct comparison nerve growth factor proved to be a strikingly more potent neuroprotective agent than VEGF. Background VEGF VEGF receptor antagonists and the .

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