TAILIEUCHUNG - Báo cáo khoa học: The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi

Neurosporaxanthin (b-apo-4¢-carotenoic acid) biosynthesis has been studied in detail in the fungus Fusarium fujikuroi. The genes and enzymes for this biosynthetic pathway are known until the last enzymatic step, the oxidation of the aldehyde group of its precursor,b-apo-4¢-carotenal. On the basis of sequence homology to Neurospora crassa | IFEBS Journal The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi Violeta Diaz-Sanchez1 Alejandro F. Estrada1 Danika Trautmann2 Salim Al-Babili2 and Javier Avalos1 1 Departamento de Genetica Facultad de Biologia Universidad de Sevilla Spain 2 Faculty of Biology Albert-Ludwigs University of Freiburg Germany Keywords apocarotenoids carotenogenesis carS mutants light regulation p-apo-4 -carotenal Correspondence J. Avalos Departamento de Genetica Universidad de Sevilla Apartado 1095 E-41080 Sevilla Spain Fax 34 954557104 Tel 34 954557110 E-mail avalos@ Present address Growth Development Biozentrum University of Basel Klingelbergstrasse 50 70 CH-4056 Basel Switzerland Received 13 May 2011 revised 17 June 2011 accepted 8 July 2011 doi Neurosporaxanthin P-apo-4 -carotenoic acid biosynthesis has been studied in detail in the fungus Fusarium fujikuroi. The genes and enzymes for this biosynthetic pathway are known until the last enzymatic step the oxidation of the aldehyde group of its precursor P-apo-4 -carotenal. On the basis of sequence homology to Neurospora crassa YLO-1 which mediates the formation of apo-4 -lycopenoic acid from the corresponding aldehyde substrate we cloned the carD gene of F. fujikuroi and investigated the activity of the encoded enzyme. In vitro assays performed with heterologously expressed protein showed the formation of neurosporaxanthin and other apocarotenoid acids from the corresponding apocarotenals. To confirm this function in vivo we generated an Escherichia coli strain producing b-apo-4 -carotenal which was converted into neurosporaxanthin upon expression of carD. Moreover the carD function was substantiated by its targeted disruption in a F. fujikuroi carotenoid-overproducing strain which resulted in the loss of neurosporaxanthin and the accumulation of P-apo-4 -carotenal its derivative P-apo-4 -carotenol and minor amounts of other .

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