TAILIEUCHUNG - Báo cáo khoa hoc : Biological characteristics of two lysines on human serum albumin in the high-affinity binding of 4Z,15Z-bilirubin-IXa revealed by phage display

4Z,15Z-bilirubin-IXa(4Z,15Z-BR), an endogenous compound that is spar-ingly soluble in water, binds human serum albumin (HSA) with high affin-ity in a flexible manner. A phage library displaying recombinant HSA domain II was constructed, after three rounds of panning against immobi-lized 4Z,15Z-BR | IFEBS Journal Biological characteristics of two lysines on human serum albumin in the high-affinity binding of 4Z 15Z-bilirubin-IXa revealed by phage display Ai Minomo1 Yu Ishima1 Ulrich Kragh-Hansen2 Victor T. G. Chuang3 Makiyo Uchida4 Kazuaki Taguchi1 Hiroshi Watanabe1 Toru Maruyama1 Hiroshi Morioka4 and Masaki Otagiri1 5 1 Department of Biopharmaceutics Graduate Schoolof PharmaceuticalSciences Kumamoto University Japan 2 Department of MedicalBiochemistry University of Aarhus Denmark 3 Schoolof Pharmacy Curtin Health Innovation Research Institute Curtin University Perth Australia 4 Department of Analyticaland BiophysicalChemistry Graduate Schoolof PharmaceuticalSciences Kumamoto University Japan 5 Drug Delivery System Research Institute Faculty of PharmaceuticalSciences Sojo University Kumamoto Japan Keywords bilirubin domain II high-affinity binding human serum albumin phage display Correspondence M. Otagiri Faculty of Pharmaceutical Sciences Sojo University 1-22-4 Ikeda Kumamoto-shi Kumamoto 860-0082 Japan Fax 81 96 362 7690 Tel 81 96 371 4150 E-mail otagirim@ Received 12 April 2011 revised 30 June 2011 accepted 24 August 2011 doi 4Z 15Z-bilirubin-IXa 4Z 15Z-BR an endogenous compound that is sparingly soluble in water binds human serum albumin HSA with high affinity in a flexible manner. A phage library displaying recombinant HSA domain II was constructed after three rounds of panning against immobilized 4Z 15Z-BR and eight clones with high affinity for the pigment were found to contain conserved basic residues such as lysine or arginine at positions 195 and 199. The wild type and two mutants K195A and K199A of whole HSA as well as stand-alone domain II were expressed in Pichia pastoris for ligand-binding studies. The binding of 4Z 15Z-BR to the K195A and K199A mutants was decreased in both whole HSA and the domain II proteins. The P-helicity conformer P-form of 4Z 15Z-BR was found to preferentially bind to the .

• Báo cáo khoa học
• Biochemistry
• molecular Biology
• biological cells
• human serum
• genetics
• Scientific reports
• Textbook of biochemistry
• Chemical basis of life
• General metabolism
• Clinical and applied biochemistry
• Applied biochemistry
• Chemistry of lipids
• Chemistry of carbohydrates
• Overview of metabolism
• Advanced biochemistry
• Steroid hormones
• Control of gene expression
• Principles of biochemistry
• Lecture Principles of biochemistry
• Biological systems
• Biochemical compounds
• Biological macromolecules
• Chemical principles
• Ebook Principles of biochemistry
• Principles of biochemistry (4th edition)
• The glyoxylate cycle
• Fatty acid catabolism
• Electron Transfer reactions
• Evolutionary foundations
• Protein interactions modulated
• Nucleic acid structure
• Ebook Plant biochemistry
• Plant biochemistry
• Plant biochemistry (Third edition)
• Several metabolic compartments
• The plant cell mechanical
• The chlorophyll molecule
• Nodule forming bacteria
• The amino acids
• The endoplasmic reticulum
• Special topics
• Harper’s illustrated biochemistry
• Replication of informational macromolecules
• Biochemistry of extracellular
• Intracellular communication
• Illustrated biochemistry
• Biochemical case histories
• The biochemistry of aging
• Signal transduction
• Hormone action
• Clinical biochemistry
• Analytical BioChemistry
• Preparation Techniques
• Analytical Chemistry
• chemical analysis
• the overall chemical analysis
• chemical analysis of materials
• General biochemistry
• biochemistry lectures
• animal biochemistry
• biochemical
• dynamic
• static biochemical
• cell membranes
• Biotechnology Biochemistry
• Algae Anatomy
• Biotechnology
• Glaucophyta
• Endosymbiosis and Origin
• Axoneme
• Lehninger principles of biochemistry
• The foundations of biochemistry
• Structure and catalysis
• The three dimensional structure of proteins
• Carbohydrates and glycobiology
• Protein function
• BRS biochemistry molecular biology
• Fuel metabolism
• Basic principles
• Basic aspects of biochemistry
• Acid–base chemistry
• Gene expression
• Synthesis of proteins
• Properties of water
• Hydrogen bonding
• Amino acids
• Amino acid structure
• Protein purification
• Cell extract
• Protein classification
• Protein 3 Dimensional structure
• Protein folding
• Protein 3 D structure
• 3 D structure
• Oxygen binding curve
• Enzyme kinetics
• Rate constants
• Catalytic Power
• Classes of enzymes
• Inhibitor kinetics
• Enzyme regulation
• Allosteric regulation
• Enzyme action
• Mechanisms of enzyme action