TAILIEUCHUNG - Báo cáo khoa học: LIN54 is an essential core subunit of the DREAM / LINC complex that binds to the cdc2 promoter in a sequence-specific manner

Recently, the conserved human LINC⁄DREAM complex has been described as an important regulator of cell cycle genes. LINC consists of a core module that dynamically associates with E2F transcription factors, p130 and the B-MYB transcription factor in a cell cycle-dependent manner. In this study, we analyzed the evolutionary conserved LIN54 subunit of LINC. | ỊFEBS Journal LIN54 is an essential core subunit of the DREAM LINC complex that binds to the cdc2 promoter in a sequence-specific manner Fabienne Schmit Sarah Cremer and Stefan Gaubatz Department of PhysiologicalChemistry I Biocenter University of Wuerzburg Germany Keywords cell cycle CXC DNA binding LIN54 LINC DREAM Correspondence S. Gaubatz Department of Physiological Chemistry I Biocenter University of Wuerzburg 97074 Wuerzburg Germany Fax 49 9a1 3184150 Tel 49 931 3184138 E-mail . Received 4 June 2009 revised 23 July 2009 accepted 5 August 2009 doi Recently the conserved human LINC DREAM complex has been described as an important regulator of cell cycle genes. LINC consists of a core module that dynamically associates with E2F transcription factors p130 and the B-MYB transcription factor in a cell cycle-dependent manner. In this study we analyzed the evolutionary conserved LIN54 subunit of LINC. We found that LIN54 is required for cell cycle progression. Protein interaction studies demonstrated that a predicted helix-coil-helix motif is required for the interaction of LIN54 with p130 and B-MYB. In addition we found that the cysteine-rich CXC domain of LIN54 is a novel DNA-binding domain that binds to the cdc2 promoter in a sequence-specific manner. We identified two binding sites for LIN54 in the cdc2 promoter one of which overlaps with the cell cycle homology region at the transcriptional start site. Gel shift assays suggested that in quiescent cells the binding of LIN54 at the cell cycle homology region is stabilized by the binding of E2F4 to the adjacent cell cycle-dependent element. Our data demonstrate that LIN54 is an important and integral subunit of LINC. Structured digital abstract MINT-7239362 LIN54 uniprotkb Q6MZP7 physically interacts MI 0915 with p130 uniprotkb Q08999 by anti tag colmmunopreclpltatlon MI 0007 MINT-7239376 LIN54 uniprotkb Q6MZP7 physically interacts MI 0915 .

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