TAILIEUCHUNG - Báo cáo y học: "Hepcidin expression in human airway epithelial cells is regulated by interferon-g"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Hepcidin expression in human airway epithelial cells is regulated by interferon-g. | Frazier et al. Respiratory Research 2011 12 100 http content 12 1 100 RESPIRATORY RESEARCH RESEARCH Open Access Hepcidin expression in human airway epithelial cells is regulated by interferon-g Marie D Frazier2 Lisa B Mamo1 Andrew J Ghio3 and Jennifer L Turi1 Abstract Background Hepcidin serves as a major regulator of systemic iron metabolism and immune function. Airway epithelial cells have an extensive interface with the environment and so must be able to respond locally to the presence of particulates infection and inflammation. Therefore we hypothesized that hepcidin is expressed in airway epithelial cells and is regulated by early phase cytokines. Methods Primary differentiated human bronchial epithelial NHBE cells were used to assess hepcidin gene expression in response to IFN-g TNF-a IL-1p and IL-6 as well as to LPS CD14. The role of the Janus Kinase-signal transducer and activator of transcription JAK-STAT pathway in IFN-g-mediated hepcidin production was assessed by measuring JAK2 phophorylation and STAT1 nuclear translocation. Inductively coupled plasma mass spectroscopy ICP-MS was used to determine whether hepcidin altered iron transport in either NHBE cells or primary alveolar macrophages. Results We demonstrate that differentiated human airway epithelial cells express hepcidin mRNA and that its expression is augmented in response to IFN-g via activation of STAT1. However while IFN-g induced hepcidin gene expression we were not able to demonstrate diminished expression of the iron export protein ferroportin Fpn at the cell surface or iron accumulation in airway epithelial in the presence of exogenous hepcidin. Conclusion These data demonstrate that airway epithelial cells express hepcidin in the lung in response to IFN-g. The presence of hepcidin in the airway does not appear to alter cellular iron transport but may serve as a protective factor via its direct antimicrobial effects. Background Hepcidin is a key regulator of .

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