TAILIEUCHUNG - Báo cáo khoa học: The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds

Thioflavin T (ThT) dye fluorescence is used regularly to quantify the for-mation and inhibition of amyloid fibrils in the presence of anti-amyloido-genic compounds such as polyphenols. However, in this study, it was shown, using three polyphenolics (curcumin, quercetin and resveratrol), that ThT fluorescence should be used with caution in the presence of such exogenous compounds. | ễFEBS Journal The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds Sean A. Hudson1 Heath Ecroyd1 2 Tak W. Kee1 and John A. Carver1 1 Schoolof Chemistry Physics The University of Adelaide Australia 2 Schoolof BiologicalSciences University of Wollongong Australia Keywords amyloid fibril thioflavin T congo red polyphenol K-casein Correspondence J. A. Carver Schoolof Chemistry Physics The University of Adelaide Adelaide SA 5005 Australia Fax 61 8 83034380 Tel 61 8 83033110 E-mail Website http Received 9 May 2009 revised 11 August 2009 accepted 13 August 2009 doi Thioflavin T ThT dye fluorescence is used regularly to quantify the formation and inhibition of amyloid fibrils in the presence of anti-amyloido-genic compounds such as polyphenols. However in this study it was shown using three polyphenolics curcumin quercetin and resveratrol that ThT fluorescence should be used with caution in the presence of such exogenous compounds. The strong absorptive and fluorescent properties of quercetin and curcumin were found to significantly bias the ThT fluorescence readings in both in situ real-time ThT assays and single time-point dilution ThT-type assays. The presence of curcumin at concentrations as low as and 1 qM was sufficient to interfere with the ThT fluorescence associated with fibrillar amyloid-b 1-42 qM and fibrillar reduced and carboxymethylated K-casein 50 qM respectively. The ThT fluorescence associated with fibrillar amyloid-b 1-42 was also biased using higher concentrations of resveratrol a polyphenol that is not spectroscopically active at the wavelengths of ThT fluorescence implying that there can be direct interactions between ThT and the exogenous compound and or competitive binding with ThT for the fibrils. Thus in all cases where ThT is used in the presence of an exogenous compound biases for .

• Báo cáo khoa học
• scientific reports
• thesis report
• medical report
• medicine
• cell proliferation
• oxidation
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes