TAILIEUCHUNG - Sputum cellularity in pulmonary tuberculosis: A comparative study between HIV-positive and -negative individuals

To compare sputum cellularity between HIV-positive and -negative individuals with pulmonary tuberculosis. A cross-sectional study was conducted in patients with pulmonary tuberculosis. Sputum samples were collected and processed within two hours after collection. The absolute number of squamous cells of a total of 400 cells was counted, as well as the absolute number (× 106 cells/ml) and percentage of eosinophils, lymphocytes, macrophages and neutrophils and total cellularity and viability were determined. Comparisons of the means of each cell type were held in a significance level of 95% (p. | Journal of AIDS and HIV Research Vol. 2 2 pp. 017 - 023 February 2010 Available online http JAHR 2010 Academic Journals Full Length Research Paper Sputum cellularity in pulmonary tuberculosis A comparative study between HIV-positive and -negative individuals Rosemeri Maurici da Silva1 Paula Stocco1 Maria Luiza Bazzo2 and Mariana Chagas2 1Universidade do Sul de Santa Catarina- Brasil. 2Universidade Federal de Santa Catarina - Brasil. Accepted 15 December 2009 To compare sputum cellularity between HIV-positive and -negative individuals with pulmonary tuberculosis. A cross-sectional study was conducted in patients with pulmonary tuberculosis. Sputum samples were collected and processed within two hours after collection. The absolute number of squamous cells of a total of 400 cells was counted as well as the absolute number x 106 cells ml and percentage of eosinophils lymphocytes macrophages and neutrophils and total cellularity and viability were determined. Comparisons of the means of each cell type were held in a significance level of 95 p . Pearson s correlation coefficient between the identified cell types was calculated. Results Assessment was performed in a cohort of 40 subjects mean age 40 years male 70 Caucasian 40 HIV-positive mean age years . Mean percentage viability in the samples was . The average value of squamous cells was . Mean percentages of cells were neutrophils eosinophils macrophages and lymphocytes. The average total cell count was x 106 cells ml. The average CD4 T-cell count in HIV-positive was cells mm3. Association of radiological patterns was present in of cases. Pearson s correlation coefficient was p between absolute counts of eosinophils and lymphocytes eosinophils and macrophages and macrophages and neutrophils. Inverse relationship was observed between the percentage of macrophages and neutrophils. There was no statistically significant .

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