TAILIEUCHUNG - The bleach method improves the detection of pulmonary tuberculosis in Laos

Sputum samples were fi rst classifi ed according to their macroscopic aspect in the laboratory. Salivary samples were immediately removed and a new sample was requested. The time between sputum collection and analysis was recorded. A 20–30 mm smear was made on a new slide with a wooden applicator. After heat fi xation, hot Ziehl-Neelsen (ZN) staining was performed: carbol fuchsin , slow heating until steaming, rinsing after 5 min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue for 1 min, rinsing and The remainder of the specimen was concentrated following the bleach method, as described by Gebre et A solution of 5% NaOCl was prepared weekly by. | INT J TUBERC LUNG DIS 13 9 1124-1129 2009 The Union The bleach method improves the detection of pulmonary tuberculosis in Laos S. Ongkhammy V. Amstutz H. Barennes Y. Buisson Institut de la Francophonie pour la Médecine tropicale Vientiane Service Fraternel d Entraide Hôpital d Attapeu Attapeu Lao Peoples Democratic Republic _SUMMARY SETTING Laos has a high prevalence of tuberculosis TB and a low prevalence of human immunodeficiency virus acquired immune-deficiency syndrome HIV AIDS . Mycobacterium tuberculosis culture is not yet available. Case finding is based on the microscopic detection of acid-fast bacilli AFB in sputum. Liquefaction and concentration using sodium hypochlorite bleach could improve its yield. OBJECTIVE To assess the gain due to the bleach method and its feasibility in Laos and to compare the classical method sputum processing direct microscopy and the bleach method for AFB detection over 3 consecutive months at a central site Vientiane and for 1 month at a peripheral site Attapeu . RESULTS Of 1675 sputum samples collected from 612 patients respectively 206 and 275 were AFB-positive by the direct and bleach methods P . an increase in smear positivity rate of 95 CI . The superiority of the bleach method was confirmed regardless of the site the aspect of the sputum and delay before analysis. This method yielded 24 more TB patients. CONCLUSION The bleach method which is very easy to use could significantly increase the yield of sputum smear microscopy for the detection of pulmonary TB in Laos. KEY WORDS tuberculosis case detection microscopy sputum sodium hypochlorite TUBERCULOSIS TB is a major public health concern in Laos where it is the seventh leading cause of 2 The population which is mainly rural 73 according to the 2005 census and poorly educated has poor access to health care facilities. The strategy of the National Tuberculosis Programme NTP is

• Y học thường thức
• Overview of resources
• Endowment and opportunities
• realizing opportunities
• Regional Synthesis
• Regional Synthesis
• Sub Regional Overviews
• Jammu and Kashmir
• Indian state of Jammu
• Diversified animal genetic resources
• Overview of diversified animal genetic resources
• Promising employment opportunities
• resources
• development
• human
• african
• south
• overview
• Technological changes
• Infrastructure development
• Inventory of resources
• Endowment opportunities
• Threats to atmospheric resources
• Demographic profile
• education teachers
• financial resources
• reflect disparities
• individual resources
• privileged backgrounds
• Biotic stress
• DUS descriptors
• Genetic resources
• Inbred lines
• Source germplasm
• property resources
• property law
• definitions
• Forms
• statutes
• Intellectual Property