TAILIEUCHUNG - Gas Chromatography and Supercritical Fluid Chromatography

Gas chromatography – chromatography using a gas as the mobile phase and a solid/liquid as a stationary phase – In GC, the analytes migrate in the gas phase, so their boiling point plays a role – GC is generally applicable to compounds with masses up to about 500 Da and with ~60 torr vapor pressure at room temp (polar functional groups are trouble) Supercritical fluid chromatography – chromatography using a supercritical fluid as the mobile phase and a solid/liquid as a stationary phase – In SFC, the analytes are solvated in the supercritical fluid – SFC is applicable to a much wider range of molecules. | VILLANOVA UNIVERSITY Gas and Supercritical Fluid Chromatography Lecture Date April 7th 2008 Gas and Supercritical Fluid Chromatography Outline - Brief review of theory - Gas Chromatography - Supercritical Fluid Extraction - Supercritical Fluid Chromatography Reading Skoog et al. - Chapter 27 Gas Chromatography - Chapter 29 Supercritical Fluid Chromatography Reading Cazes et al. - Chapter 23 Gas Chromatography - Chapter 24 Supercritical Fluid Chromatography 1 GC and SFC Very Basic Definitions Gas chromatography - chromatography using a gas as the mobile phase and a solid liquid as a stationary phase - In GC the analytes migrate in the gas phase so their boiling point plays a role - GC is generally applicable to compounds with masses up to about 500 Da and with 60 torr vapor pressure at room temp polar functional groups are trouble Supercritical fluid chromatography - chromatography using a supercritical fluid as the mobile phase and a solid liquid as a stationary phase - In SFC the analytes are solvated in the supercritical fluid - SFC is applicable to a much wider range of molecules Review of Chromatography Important concepts equations to remember Selectivity a KB IKA Retention volume V iF n Column separation performance Plates Hv LIH Linear velocity of mobile phase 2 GC Theory Mobile-phase flow rates are much higher in GC pressure drop is much less for a gas The effect of mobile-phase flow rate on the plate height H is dramatic - Lower plate heights yield better chromatography - However much longer columns can be used with GC

• Hoá dầu
• Chromatography
• Gas Chromatography
• Supercritical Fluid
• Chromatography A
• Cation exchange chromatography
• Frontal chromatography
• Continuous loading chromatography
• Overloaded chromatography
• Single use chromatography
• Anion exchange chromatography
• Polishing chromatography
• Monoclonal antibodies
• Pyrolysis gas chromatography
• Size exclusion chromatography
• Nitroxide mediated radical polymerization
• Reversible addition fragmentation chain transfer
• Phenolic compound
• Supercritical fluid chromatography
• Trapping capacity
• Two dimensional chromatography
• Method transfer
• Reversed phase liquid chromatography
• Liquid chromatography
• Mass spectrometry
• Synthetic peptides
• Mixed mode liquid chromatography
• Hydrophilic interaction liquid chromatography
• Stationary phase
• Cycle durability tests
• Mineral oil characterization
• Argentation chromatography
• Mineral oil aromatic hydrocarbons
• Vacuum ultraviolet detection
• Mineral oil analysis
• Parallel detection
• Hydrophilic interaction chromatography
• Nuclear magnetic resonance
• HILIC elution conditions
• Glycoprotein glycans
• Online cleanup liquid chromatography
• High performance liquid chromatography
• Solid phase extraction
• Two dimensional liquid chromatography
• Metal oxide chromatography
• Alzheimer’s disease
• Phosphorylated tau
• Nuclear forensics
• Extraction chromatography
• Extraction chromatography resins
• Decontamination factor
• Chromatography Hyphenated
• Degradation Phenomena
• Organic Reactions
• Recent Strategies
• Cyclodextrin electrokinetic chromatography
• Chiral separation
• Dual systems
• Dubsky’s model
• Electrokinetic chromatography
• Phosphorylated organic molecules
• Lipid biomarkers
• Reversed phase
• Butylene terephthalate
• Ion exchange chromatography
• Monoclonal antibody
• Mab biosimilars
• Poly propylene oxide
• Functionality type distribution
• Two dimensional distributions
• Cyclodextrin chiral ionic liquids dual systems
• Enantiomeric separation
• Capillary electrokinetic chromatography
• Extracellular vesicles
• Heparin affinity chromatography
• Size exclusion chromatography
• Clarified conditioned media
• Column Chromatography
• Drug Discovery
• Bioactivity Analysis
• Affinity Chromatography
• General Description
• Residue analysis
• Orthosulfamuron herbicide
• Fatty rice
• Liquid chromatography–tandem mass spectrometry
• Chromatography–tandem mass spectrometry