TAILIEUCHUNG - báo cáo khoa học: "Use of silver nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Use of silver nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins | Lara et al. Journal of Nanobiotechnology 2011 9 38 http content 9 1 38 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Use of silver nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins Humberto H Lara1 Liliana Ixtepan-Turrent2 Elsa N Garza Trevino2 and Dinesh K Singh1 Abstract Background HIV AIDS pandemic is a worldwide public health issue. There is a need for new approaches to develop new antiviral compounds or other therapeutic strategies to limit viral transmission. The envelope glycoproteins gp120 and gp41 of HIV are the main targets for both silver nanoparticles AgNPs and neutralizing antibodies. There is an urgency to optimize the efficiency of the neutralizing antibodies NABs . In this study we demonstrated that there is an additive effect between the four NABs and AgNPs when combined against cell-associated HIV-1 infection in vitro Results Four NABs Monoclonal antibody to HIV-1 gp41 126-7 HIV-1 gp120 Antiserum PB1 Sub 2 HIV-1 gp120 Antiserum PB1 HIV-1 gp120 Monoclonal Antibody F425 B4e8 with or without AgNPs of 30-50 nm in size were tested against cell free and cell-associated HIVIIIB virus. All NABs inhibited HIV-1 cell free infection at a dose response manner but with AgNPs an antiviral additive effect was not achieved Although there was no inhibition of infection with cell-associated virus by the NABs itself AgNPs alone were able to inhibit cell associated virus infection and more importantly when mixed together with NABs they inhibited the HIV-1 cell associated infection in an additive manner. Discussion The most attractive strategies to deal with the HIV problem are the development of a prophylactic vaccine and the development of effective topical vaginal microbicide. For two decades a potent vaccine that inhibits transmission of infection of HIV has been searched. There are vaccines that elicit NABs but none of them has the efficacy to .

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