TAILIEUCHUNG - Báo cáo khoa học: Neuropeptide Y expression and function during osteoblast differentiation – insights from transthyretin knockout mice

To better understand the role of neuropeptide Y (NPY) in bone homeosta-sis, as its function in the regulation of bone mass is unclear, we assessed its expression in this tissue. By immunohistochemistry, we demonstrated, both at embryonic stages and in the adult, that NPY is synthesized by osteoblasts, osteocytes, and chondrocytes. | ỊFEBS Journal Neuropeptide Y expression and function during osteoblast differentiation - insights from transthyretin knockout mice Ana F. Nunes1 2 z Marcia A. Liz1 Filipa Franquinho1 Liliana Teixeira3 Vera Sousa1 Chantal Chenu4 Meriem Lamghari3 f and Monica M. Sousa1 f 1 Nerve Regeneration IBMC - Institute de Biologia Molecular e Celular Universidade do Porto Portugal 2 ICBAS Universidade do Porto Portugal 3 INEB - Instituto de Engenharia Biomedica Divisao de Biomateriais Universidade do Porto Portugal 4 Department of Veterinary Basic Sciences The RoyalVeterinary College London UK Keywords amidated neuropeptide bone marrow stromalcells bone mass NPY osteoblastic differentiation Correspondence M. M. Sousa IBMC Rua Campo Alegre 823 4150-180 Porto Portugal Fax 351 22 6099157 Tel 351 22 6074900 E-mail msousa@ Website http nerve Present address Faculty of Pharmacy University of Lisbon Portugal fThese authors contributed equally to this work Received 12 November 2009 revised 3 November 2009 accepted 5 November 2009 doi To better understand the role of neuropeptide Y NPY in bone homeostasis as its function in the regulation of bone mass is unclear we assessed its expression in this tissue. By immunohistochemistry we demonstrated both at embryonic stages and in the adult that NPY is synthesized by osteoblasts osteocytes and chondrocytes. Moreover peptidylglycine a-am-idating monooxygenase the enzyme responsible for NPY activation by amidation was also expressed in these cell types. Using transthyretin TTR KO mice as a model of augmented NPY levels we showed that this strain has increased NPY content in the bone further validating the expression of this neuropeptide by bone cells. Moreover the higher ami-dated neuropeptide levels in TTR KO mice were related to increased bone mineral density and trabecular volume. Additionally RT-PCR analysis established that NPY is not only expressed in MC3T3-E1 osteoblastic

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