TAILIEUCHUNG - Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family

The crystal structure of flavoredoxin fromDesulfovibrio vulgarisMiyazaki F was determined at A˚ resolution and its ferric reductase activity was examined. The aim was to elucidate whether flavoredoxin has structural similarity to ferric reductase and ferric reductase activity, based on the sequence similarity to ferric reductase from Archaeoglobus fulgidus. | ễFEBS Journal Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family Naoki Shibata1 Yasufumi Ueda1 Daisuke Takeuchi2 Yoshihiro Haruyama2 Shuichi Kojima3 Junichi Sato4 Youichi Niimura4 Masaya Kitamura2 and Yoshiki Higuchi1 1 Department of Life Science University of Hyogo Japan 2 Department of Applied Chemistry and Bioengineering Osaka City University Japan 3 Institute for Biomolecular Science Gakushuin University Tokyo Japan 4 Department of Bioscience Tokyo University of Agriculture Japan Keywords crystal structure electron transfer flavin mononucleotide flavin reductase family sulfate-reducing bacterium Correspondence M. Kitamura Department of Applied Chemistry and Bioengineering Graduate School of Engineering Osaka City University 3-3-138 Sugimoto Sumiyoshi-ku Osaka 558-8585 Japan Fax 81 666 05 2769 Tel 81 666 05 3091 E-mail kitamura@ Y. Higuchi Department of Life Science Graduate Schoolof Life Science University of Hyogo 3-2-1 Koto Kamigori-cho Ako-gun Hyogo 678-1297 Japan Fax 81 791 58 0177 Tel 81 791 58 0179 E-mail hig@ Database The coordinates and structure factor data have been deposited in the PDB under the accession number 2D5M. The nucleotide and amino acid sequence data may be found in the DDBJ EMBL and GenBank sequence databases under the accession numbers AB214904 and BAD99043 respectively Received 30 March 2009 revised 28 May 2009 accepted 29 June 2009 doi The crystal structure of flavoredoxin from Desulfovibrio vulgaris Miyazaki F was determined at A resolution and its ferric reductase activity was examined. The aim was to elucidate whether flavoredoxin has structural similarity to ferric reductase and ferric reductase activity based on the sequence similarity to ferric reductase from Archaeoglobus fulgidus. As expected flavoredoxin shared a common .

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