TAILIEUCHUNG - Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion

The structure of the reduced form of cytochromec6 from the mesophilic cyanobacteriumSynechococcussp. PCC 7002 has been determined at A ˚ and refined to an R-factor of . This protein is unique among all known cytochromesc6, owing to the presence of an unusual seven-residue insertion, KDGSKSL(44–50), which differs from the insertion found in the recently discovered plant cytochromesc6A . | Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion Wojciech Bialek1 Szymon Krzywda2 Mariusz Jaskolski2 3 and Andrzej Szczepaniak1 1 Department of Biophysics Faculty of Biotechnology University of Wroclaw Poland 2 Department of Crystallography Faculty of Chemistry A. Mickiewicz University Poznan Poland 3 Center for Biocrystallographic Research Institute of Bioorganic Chemistry Polish Academy of Sciences Poznan Poland Keywords cyanobacteria cytochrome high-resolution structure photosynthesis Synechococcus sp. PCC 7002 Correspondence A. Szczepaniak Department of Biophysics Faculty of Biotechnology University of Wroclaw Przybyszewskiego 63 77 51-148 Wroclaw Poland Fax 48 71 3756234 Tel 48 71 3756236 E-mail M. Jaskolski Department of Crystallography Faculty of Chemistry A. Mickiewicz University Grunwaldzka 6 60-780 Poznan Poland Fax 48 61 829 1505 Tel 48 61 829 1274 E-mail mariuszj@ Database Atomic coordinates and structure factors are available from the Protein Data Bank under the accession code 3DR0 The structure of the reduced form of cytochrome c6 from the mesophilic cyanobacterium Synechococcus sp. PCC 7002 has been determined at A and refined to an R-factor of . This protein is unique among all known cytochromes c6 owing to the presence of an unusual seven-residue insertion KDGSKSL 44-50 which differs from the insertion found in the recently discovered plant cytochromes c6A. Furthermore the present protein is unusual because of its very high content 36 of the smallest residues glycine and alanine . The structure reveals that the overall fold of the protein is similar to that of other class I c-type cytochromes despite the presence of the specific insertion. The insertion is located within the most variable region of the cytochrome c6 sequence . between helices II and III. The first six residues KDGSKS 44-49 form a loop whereas the last residue Leu50 .

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