TAILIEUCHUNG - Báo cáo khoa học: "Micropropagation of Paulownia taiwaniana from mature tissues"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Micropropagation of Paulownia taiwaniana from mature tissues. | 165s Ann. Sci. For. 1989 46 suppl. 165s-167s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Micropropagation of Paulownia taiwaniana from mature tissues . Yang . Chang and . Ho Silviculture Division Taiwan Forestry Research institute 53 Nan-Hai Rd. Taipei 10728 Taiwan . Introduction Paulownia taiwaniana is an important agroforestry species in Taiwan. However witches broom has impaired the reforestation program for many years. The search for disease-free individuals in infected plantations and then multiplying them by means of shoot-tip culture is part of our pest management program. This paper reports the appropriate techniques to produce disease-free plants from mature tissues of p. taiwaniana. Materials and Methods The apices of small branches and their lateral shoots which emerged after the removal of branch tips were excised from an 8 yr old tree of p. taiwaniana. Both meristem tissues cm long were cleaned under running tap water for 30 min followed by sterilization in 70 ethanol for 30 s and then in sodium hypochlorite in a supersonic vibrator for 10 min. Finally the tissues were washed 3 times in sterile distilled water. The sterilized explants were first cultured on solid and liquid Murashige and Skoog 1962 MS basic media with full 1 2 and 1 3 strength and supplemented with mg l 6-benzyl- aminopurine BA or kinetin for the induction of multishoots. The individual stems separated from multishoots were then transferred onto the same MS basic medium but supplemented with 0-4 mg l 2-naphthalene acetic acid NAA or indole-3-butyric acid IBA for root formation. All cultures were incubated at 25 2 c with a 10 h photoperiod and light intensity of 65-75 pmols-i-nr2. Results and Discussion After 7 days of incubation in MS medium the survival rate of lateral shoots was 99 while branch apices became brown and finally died indicating that rejuvenation by means of trimming branches may improve the survival and overcome tissue .

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