TAILIEUCHUNG - Báo cáo khoa học: Hansenula polymorpha pex11 cells are affected in peroxisome retention

We have cloned and characterized theHansenula polymorpha PEX11gene. Our morphological data are consistent with previous observations that peroxisome proliferation can be regulated by modulating Pex11p levels. Surprisingly,pex11cells also showed a defect in peroxisome retention in mother cells during vegetative cell reproduction. | Hansenula polymorpha pex11 cells are affected in peroxisome retention Arjen M. Krikken1 Marten Veenhuis1 2 and Ida J. van der Klei1 2 1 Molecular CellBiology Groningen Biomolecular Sciences and Biotechnology Institute GBB University of Groningen Haren The Netherlands 2 Kluyver Centre for Genomics of IndustrialFermentation Delft The Netherlands Keywords inheritance Inp1p peroxisome Pex11p yeast Correspondence I. J. van der Klei . Box 14 9750AA Haren The Netherlands Fax 31 50 363 8280 Tel 31 50 363 2179 E-mail Received 7 November 2008 revised 22 December 2008 accepted 30 December 2008 doi We have cloned and characterized the Hansenula polymorpha PEX11 gene. Our morphological data are consistent with previous observations that peroxisome proliferation can be regulated by modulating Pex11p levels. Surprisingly pexll cells also showed a defect in peroxisome retention in mother cells during vegetative cell reproduction. Until now Saccharo-myces cerevisiae Inp1p has been the only peroxisomal protein that has been shown to play a role in the organelle retention process. H. polymorpha inp1 cells are also affected in peroxisome retention like pex11 cells. We show by time-lapse imaging that Inp1-green fluorescent protein localization varies during the cell cycle and that the protein is normally recruited to peroxisomes in pex11 cells. Taken together our data show that H. polymorpha Pex11p is not only important for peroxisome proliferation but is also required for proper peroxisome segregation during cell division. Peroxisomes are single-membrane-bound highly dynamic organelles that have crucial functions in eukaryotic cells 1 . Peroxisome loss or malfunction in human leads to severe diseases that may lead to an early death . Zellweger syndrome 2 . For almost 20 years it was assumed that peroxisomes exclusively multiply by growth and division 3 . However several groups unequivocally demonstrated that peroxisomes .

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