TAILIEUCHUNG - Báo cáo y học: "Regulation and localization of endogenous human tristetraprolin"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Regulation and localization of endogenous human tristetraprolin. | Arthritis Research Therapy Vol 5 No 4 Fairhurst et al. Research article Open Access Regulation and localization of endogenous human tristetraprolin Anna-Marie Fairhurst1 6 John E Connolly2 6 Katharine A Hintz3 Nicolas J Goulding1 Athos J Rassias4 Mark P Yeager4 William Rigby5 2 Paul K Wallace2 1William Harvey Research Institute University of London London UK 2Department of Microbiology and Immunology Dartmouth Medical School Lebanon NH USA 3Department of Physiology Dartmouth Medical School Lebanon NH USA 4Department of Anesthesiology Dartmouth Medical School Lebanon NH USA 5Department of Medicine Dartmouth Medical School Lebanon NH USA 6These authors contributed equally to this work. Corresponding author Anna-Marie Fairhurst e-mail fairhursta@ Received 1 Feb 2003 Revisions requested 5 Mar 2003 Revisions received 28 Mar 2003 Accepted 11 Apr 2003 Published 15 May 2003 Arthritis Res Ther 2003 5 R214-R225 DOI ar778 2003 Fairhurst et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Tumor necrosis factor TNF has been implicated in the development and pathogenicity of infectious diseases and autoimmune disorders such as septic shock and arthritis. The zinc-finger protein tristetraprolin TTP has been identified as a major regulator of TNF biosynthesis. To define its intracellular location and examine its regulation of TNF a quantitive intracellular staining assay specific for TTP was developed. We establish for the first time that in peripheral blood leukocytes expression of endogenous TTP is confined to the cytoplasm. Baseline expression of TTP was higher in monocytes than in lymphocytes or neutrophils. After in vitro incubation with lipopolysaccharide LPS leukocyte TTP levels increased rapidly peaking after .

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