TAILIEUCHUNG - Báo cáo khoa học: "gelling agents on growth, mineral composition and naphthoquinone content of in vitro explants of hybrid walnut tree (Juglans regia x Juglans nigra)"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "gelling agents on growth, mineral composition and naphthoquinone content of in vitro explants of hybrid walnut tree (Juglans regia x Juglans nigra). | AnnSciFor 1993 50 177-186 Elsevier INRA 177 Original article Effects of gelling agents on growth mineral composition and naphthoquinone content of in vitro explants of hybrid walnut tree Juglans regia X Juglans nigra E Barbas 1 c Jay-Allemand p Doumas 1 s Chaillou 2 D Cornu 1 INRA Station d Amelioration des Arbres Forestiers 45160 Olivet 2INRA Laboratoire du Métabolisme et de la Nutrition des Plantes 78000 Versailles France Received 15 May 1992 accepted 22 October 1992 Summary Gelling agents affect growth of walnut in vitro cultured shoots. Gelrite promoted shoot elongation and bud production whereas agar inhibited growth induced mature leaf formation and necroses. The 2 gelling agents differed significantly in mineral content. They altered the chemical composition of the medium as well as that of the explants. A pronounced accumulation of Na and several microelements was observed in leaves after 16 d of culture on agar probably due to a disturbance in the K selectivity mechanism and membrane permeability. Moreover on agar the level of hydrojuglone glucoside a marker of juvenility in walnut decreased drastically in the callus. Mineral element accumulation and decrease of hydrojuglone glucoside were evident after growth inhibition indicating that they are a result rather than a cause of this inhibition. Lack of growth mature foliar morphology Na and microelement accumulation and hydrojuglone glucoside decline support the hypothesis that agar accelerates the ageing of in vitro propagated walnut trees. Juglans I micropropagation gelling agent I mineral composition Resume Effets des agents de solidification du milieu de culture sur la croissance la composition minérale et la teneur en hydrojuglone glucoside des explants de noyer hybride culti-vés in vitro. Les agents de solidification influent sur la croissance des pousses du noyer cultivées in vitro fig 1 . La gelrite a un effet benefique sur elongation des explants et la production de bourgeons tandis que 1 agar .

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