TAILIEUCHUNG - Báo cáo khoa học: "Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture. | 295s Ann Sci For 1993 50 Suppl 1 295s-307s Elsevier INRA Review article Vegetative propagation of oak Quercus robur and Q petraea by cutting and tissue culture V Chalupa Faculty of Forestry University of Agricultural Sciences 16521 Praha 6-Suchdol Czech Republic Summary The potential of cuttings of Quercus robur and o petraea to form adventitious roots decreased rapidly with increasing plant age. The rooting ability of older plants was increased by hedging. Hedging of stock plants offers an effective technique for the production of cuttings with high rooting potential stock plant environment markedly affected rooting of leafy cuttings. A high percentage of cuttings collected from plants grown under continuous light rooted. Vigorous plants were produced from cuttings which rooted quickly and were capable of rapid shoot growth immediately after rooting. Shoot growth of rooted cuttings was stimulated in suitable environmental conditions by sufficient mineral nutrition. Rooted cuttings which formed new long shoots and wintered in rooting medium in the same place in an unheated greenhouse exhibited high survival rates. For tissue culture propagation 2 methods were used micropropagation by axillary shoot multiplication and by somatic embryogenesis. Axillary shoot multiplication was stimulated on low salt media BTM or woody plant medium WPM supplemented with a low concentration of benzylaminopurine BAP or N-benzyl -9- 2-tetrahydropyranyl adenine BPA mg -1 . Rooting of microshoots was achieved in vitro and was also successful under non-sterile conditions in a rooting mixture of peat and perlite. The field growth of micropropagated trees was comparable to that of control seedlings. Embryogenic cultures were initiated from immature zygotic embryos of Q petraea cultured on modified Schenk and Hildebrandt SH medium supplemented with BAP 1mg r1 . The majority of embryogenic cultures produced somatic embryos. The conversion of somatic embryos into plantlets was achieved

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