TAILIEUCHUNG - Báo cáo khoa học: " Mating system in a clonal Douglas fir (Pseudotsuga menziesii (Mirb) Franco) seed orchard. I. Gene diversity and structure"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: " Mating system in a clonal Douglas fir (Pseudotsuga menziesii (Mirb) Franco) seed orchard. I. Gene diversity and structure. | Ann Sci For 1995 52 201-211 Elsevier INRA 201 Original article Mating system in a clonal Douglas fir Pseudotsuga menziesii Mirb Franco seed orchard. I. Gene diversity and structure D Prat T Caquelard Laboratoire INRA-ENGREF de sciences forestieres 14 rue Girardet 54042 Nancy cedex France Received 15 February 1994 accepted 15 July 1994 Summary The clonal seed orchard studied consisted of 60 clones phenotypically selected in Douglas fir stands planted in France during the first half of the twentieth century. The genotype of every clone was characterised using isozyme techniques. Seven enzyme systems were studied 1 was monomorphic and 9 polymorphic loci were analysed. Seven loci from 5 enzyme systems were sufficient for genetic identification of each clone. Rare alleles were noticed at 7 of the 9 polymorphic loci studied. Three rare alleles were used to assess the selfing rate of individual ramets but 2 of them might have led to erroneous results because of their selective disadvantage. The individual selfing rates assessed from the rare allele transmission by pollen were low 2-5 . The gene diversity in pollen received by a single ramet was not representative of that of the seed orchard and was not similar to that expected according to the seed orchard composition. Commercial seed crop exhibited a higher fixation index than that related to selfing rate and a significant deviation from the allelic frequencies expected in the orchard. isozyme I mating system rare allele selfing Pseudotsuga menziesii Douglas fir Resume Regime de reproduction dans un verger à graines de Douglas Pseudotsuga men-ziesii Mirb Franco . I. structure et variabilité génétique. Le verger à graines étudié est constitué de 60 clones sélectionnés phénotypiquement dans des peuplements frangais de Douglas plantés au cours de la premiere moitié du XXs siècle. Chaque clone a été caractérisé par son profil enzymatique. Sept systèmes enzymatiques ont été révélés un seul s est montré monomorphe 9 locus .

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