TAILIEUCHUNG - Báo cáo khoa học: "Metabolism of isopentenyladenosine in the roots of Norway spruce seedlings exposed to nutritive stress"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài:"Metabolism of isopentenyladenosine in the roots of Norway spruce seedlings exposed to nutritive stress. | Ann Sci For 1995 52 57-65 Elsevier INRA 57 Original article Metabolism of isopentenyladenosine in the roots of Norway spruce seedlings exposed to nutritive stress K von Schwartzenberg t M Bonnet-Masimbert p Doumas INRA Centre de Recherche Orleans station d Amelioration des Arbres Forestiers 45160 Ardon France Received 9 June 1993 accepted 8 June 1994 Summary Seedlings of Norway spruce Picea abies were grown on a low nutrient medium containing Al3 ions mM stress and a rich medium that was suitable for spruce and lacked Al3 control . After feeding with tritiated isopentenyladenosine via the roots the metabolism of cytokinins in the roots of stressed and control plants was compared. HPLC radioactivity profiles of root extracts showed that isopentenyladenosine was mainly degraded to isopentenyladenine- and adenine adenosine-like compounds. Stressed and non-stressed seedlings clearly differed with respect to the distribution of radioactivity for the different metabolites. The measurements showed that the degradation of isopentenyl-adenosine was strongly reduced in the roots of the stressed seedlings. Results are discussed with regard to the levels of endogenous cytokinins measured in spruce affected by the novel type of forest decline. Picea abies I cytokinin metabolism radiolabelled cytokinin forest decline Resume Métabolisme de risopentényladénosine dans les racines d epicea soumis à un stress nutritif. De jeunes plantules d epicea Picea abies ont été plantés sur un milieu pauvre conte-nant des ions AP 0 8 mM stress et sur un milieu sans Al3 bien fourni en nutriments témoin . Après marquage avec de 1 isopentényladénosine trítiée via les racines le métabolisme des cytokinines dans les racines a été compare Chez les plants témoins et Chez les plants stresses. Les profils de radioactivité obtenus après HPLC à partir des extraits racinaires montrent que 1 isopentényladéno-sine est principalement degrade en isopentényladénosine et adenine OU adenosine. Les plantules .

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