TAILIEUCHUNG - Báo cáo khoa học: "Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP)"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP). | 66s Ann Sci For 1993 50 Suppl 1 66S-69S Elsevier INRA Note Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism RFLP K Burg 1 M Zechmeister-Machhart 2 J Glossl 2 J Schmidt1 Austrian Research Center Department of Biotechnology Seibersdorf 2 Center of Applied Genetics University of Bodenkultur Vienna Austria Summary Petunia hybrida chloroplast cp DNA probes were used to find restriction fragment length polymorphisms RFLPs in the cp DNA of the oak species Quercus robur and Quercus pe-traea. Five individuals have been analysed 2 Q robur and 3 o petraea with 18 different restriction enzymes and with 7 p hybrida cp DNA probes. Only 2 probes P6 and P8 I detected polymorphisms probe P6 detected 4 polymorphisms with the restriction enzymes Aval Bg ll c al and Xbal while probe P8 detected a Bg ll polymorphism. oak I chloroplast restriction fragment length polymorphism RFLP Resume Polymorphisme de longueur des fragments de restriction de I ADN chloroplas-tique Chez les chênes. Des sondes du genome chloroplastique de Petunia hybrida ont été utilisées pour la recherche de polymorphisme de longueur de fragment de restriction dans I ADN chloroplas-tique cp de Quercus robur et Quercus petraea. LADNcp de 5 arbres 2 Q robur et 3 Q petraea a ổté digéré par 18 enzymes de restriction et hybridé avec 7 sondes de p hybrida. Deux sondes seule-ment ont révélé du polymorphisme P6 et P8 P6 avec les enzymes de restriction Aval Bgl Cla ef Xba P8 avec I enzyme Bgl . Quercus chloroplaste polymorphisms de longueur de fragment de restriction RFLP INTRODUCTION Climatic variations as well as human activities eg environmental pollution greatly influence natural ecosystems frequently restricting the habitat reproductive potential and number of individuals of many species. These restrictions may reduce genetic variability within populations which may in turn diminish the capacity of populations to respond to new selective pressures. Therefore we need better insight .

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