TAILIEUCHUNG - Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine. | Int. J. Med. Sci. 2008 5 189 International Journal of Medical Sciences ISSN 1449-1907 2008 5 4 189-196 Ivyspring International Publisher. All rights reserved Research Paper Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line Evidence for an Anti-Estrogen Effect of Iodine Frederick R. Stoddard II 1 2 Ari D. Brooks1 Bernard A. Eskin3 and Gregg J. Johannes2 1. Department of Surgery Drexel University College of Medicine Philadelphia PA 19102 USA 2. Department of Pathology and Laboratory Medicine Drexel University College of Medicine Philadelphia PA 19102 USA 3. Department of OB GYN Drexel University College of Medicine Philadelphia PA 19102 USA Correspondence to Gregg J. Johannes . Drexel University College of Medicine 245 N. 15th Street M S 435 Philadelphia PA 19102. Ph 215 762-8173 Fax 215 246-5918 Email Received Accepted Published The protective effects of iodine on breast cancer have been postulated from epidemiologic evidence and described in animal models. The molecular mechanisms responsible have not been identified but laboratory evidence suggests that iodine may inhibit cancer promotion through modulation of the estrogen pathway. To elucidate the role of iodine in breast cancer the effect of Lugol s iodine solution 5 I2 10 KI on gene expression was analyzed in the estrogen responsive MCF-7 breast cancer cell line. Microarray analysis identified 29 genes that were up-regulated and 14 genes that were down-regulated in response to iodine iodide treatment. The altered genes included several involved in hormone metabolism as well as genes involved in the regulation of cell cycle progression growth and differentiation. Quantitative RT-PCR confirmed the array data demonstrating that io-dine iodide treatment increased the mRNA levels of several genes involved in estrogen metabolism CYP1A1 CYP1B1 and AKR1C1 while decreasing the levels of the estrogen responsive genes TFF1 and

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