TAILIEUCHUNG - Báo cáo khoa học: " Identification of PCR-based markers linked to wood splitting in Eucalyptus grandis"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Identification of PCR-based markers linked to wood splitting in Eucalyptus grandis. | Ann. For. Sci. 59 2002 675-678 INRA EDP Sciences 2002 DOI forest 2002055 675 Note Identification of PCR-based markers linked to wood splitting in Eucalyptus grandis Eugenia Barrosa Steve Verrynb and Marianne Hettaschb aCSIR-Bio Chemtek 395 Pretoria 0001 South Africa bCSIR-Environmentek 395 Pretoria 0001 South Africa Received 5 July 2001 accepted 17 April 2002 Abstract - Wood splitting is a defect in eucalyptus which results in considerable losses when converting logs to solid wood products. Commonly in forestry molecular markers are identified through studying pedigrees from a single cross. This limits the application of these markers to that population. Here we report the identification of putative molecular markers linked to wood splitting in an open-pollinated Eucalyptus grandis population. Although the power to detect molecular markers in this population is low the resultant markers are likely to be more robust and be transferred to non-related populations. Bulked segregant analysis was used in the identification of markers from high and low splitting individuals that were selected by means of backward selection using Best Linear Prediction. The bulks were screened for differences using amplified fragment length polymorphic and random amplified polymorphic DNA primers. Following regression analysis one putative sequence characterized amplified region has been linked to splitting. wood splitting molecular markers Eucalyptus grandis BLP Résumé - Identification de marqueurs PCR lies au defaut de fente du bois chez Eucalyptus grandis. La fente du bois est un défaut chez l eu-calyptus qui entraĩne des pertes considérables lors de la transformation du bois en produits finis massifs. Classiquement chez les arbres forestiers les marqueurs moléculaires sont identifiés en étudiant les descendants d un seul croisement. Ceci limite l application de ces marqueurs à cette seule population. Dans cette étude nous avons cherché à identifier des marqueurs

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