TAILIEUCHUNG - Handbook of Microbiological Media, Fourth Edition part 53

Handbook of Microbiological Media, Fourth Edition part 53 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Desulfobacterium Medium with Lactate 515 Solution F Composition per Preparation of Solution F Add Na2S-9H2O to distilled deionized water and bring volume to . Gas under 100 N2 for 20 min. Cap with a rubber stopper. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Solution G Composition per Preparation of Solution G Add Na2S2O4 to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Aseptically gas under 100 N2 for 20 min. Prepare solution freshly. Preparation of Medium To of cooled sterile solution A aseptically and anaerobically add in the following order of sterile solution B of sterile solution C of sterile solution D of sterile solution E and of sterile solution F Mix thoroughly. Immediately prior to inoculation aseptically and anaerobically add of sterile solution G Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Desulfobacterium auto-trophicum. Desulfobacterium Medium with Lactate Composition per Solution Solution Solution D Wolfe s vitamin solution . Solution Solution B Trace elements solution SL-10 . Solution Solution pH at 25 C Solution A Composition per Lactic acid sodium Preparation of Solution A Prepare and dispense solution anaerobically under 80 N2 20 CO2. Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Continue boiling until resazurin turns colorless indicating reduction. Cap with rubber stoppers. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Solution B Trace Elements Solution SL-10 Composition per liter .

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