TAILIEUCHUNG - báo cáo hóa học: " Nogo receptor is involved in the adhesion of dendritic cells to myelin"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Nogo receptor is involved in the adhesion of dendritic cells to myelin | McDonald et al. Journal of Neuroinflammation 2011 8 113 http content 8 1 113 JIOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Nogo receptor is involved in the adhesion of dendritic cells to myelin l-sizz I h I r z I z l 1 If zl o Fz I h r 2 E I z z I z IX z zz 3 D I IZ 1 I Z t z z c r ht A I Z t zz iFz z3 Dzv I z zd I zFi v 4 h ZI FỈ z z E D- Z-. z- f I Z it A t3 Claire L McDonald Karin Steinbach Florian Kern Rudiger schweigreiter Roland Martin Christine E Bandtlow and Markus Reindl1 Abstract Background Nogo-66 receptor NgR1 and its structural homologue NgR2 are binding proteins for a number of myelin-associated inhibitory factors. After neuronal injury these inhibitory factors are responsible for preventing axonal outgrowth via their interactions with NgR1 and NgR2 expressed on neurons. In vitro cells expressing NgR1 2 are inhibited from adhering to and spreading on a myelin substrate. Neuronal injury also results in the presence of dendritic cells DCs in the central nervous system where they can come into contact with myelin debris. The exact mechanisms of interaction of immune cells with CNS myelin are however poorly understood. Methods Human DCs were differentiated from peripheral blood monocytes and mouse DCs were differentiated from wild type and NgR1 NgR2 double knockout bone marrow precursors. NgR1 and NgR2 expression were determined with quantitative real time PCR and immunoblot and adhesion of cells to myelin was quantified. Results We demonstrate that human immature myeloid DCs express NgR1 and NgR2 which are then down-regulated upon maturation. Human mature DCs also adhere to a much higher extent to a myelin substrate than immature DCs. We observe the same effect when the cells are plated on Nogo-66-His binding peptide for NgR1 but not on control proteins. Mature DCs taken from Ngr1 2 knockout mice adhere to a much higher extent to myelin compared to wild type mouse DCs. In addition Ngr1 2 knockout had no effect on in .

TÀI LIỆU LIÊN QUAN
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.