TAILIEUCHUNG - Cancer Cell Culture Methods and Protocols

Cell culture is practiced extensively throughout the world today. The techniques required to allow cells to grow and be maintained outside the body have been developed throughout the 20th century. In the 50 years since the publication of the first human cancer cell line, HeLa (1), thousands of cell lines representing most of the spectrum of human cancer have been derived. These have provided tools to study in depth the biochemistry and molecular biology associated with individual cancer types and have helped enormously in our understanding of normal as well as cancer cell physiology. Although some caution is required in interpreting data obtained by studying cells in vitro,. | M E T H O D S I N M O L E C U L A R M E D I C I N ETM 1 Basic Principles of Cancer Cell Culture Simon P. Langdon 1. Introduction Cell culture is practiced extensively throughout the world today. The techniques required to allow cells to grow and be maintained outside the body have been developed throughout the 20th century. In the 50 years since the publication of the first human cancer cell line HeLa 1 thousands of cell lines representing most of the spectrum of human cancer have been derived. These have provided tools to study in depth the biochemistry and molecular biology associated with individual cancer types and have helped enormously in our understanding of normal as well as cancer cell physiology. Although some caution is required in interpreting data obtained by studying cells in vitro it has allowed investigation of a complex disease such as cancer to be simplified to its component parts. The aim of this chapter is to introduce some of the basic concepts involved in the practice of cell culture. 2. Evolution of Cancer Cell Culture The science of cell and tissue culture has evolved steadily throughout the last century and its origins can be traced back to 1885 see Table 1 . In that year Wilhelm Roux reported that the medullary plate of a chick embryo could be maintained in saline solution for several days. Many of the early experiments used material derived from amphibians as it was cold blooded and often demonstrated tissue regeneration. In 1887 Arnold demonstrated that frog lymphocytes could migrate and survive in saline. Soon after in 1898 the first experiment using human tissue was reported when Ljunggren showed that human skin could survive in vitro if placed in ascitic fluid. With the turn of the century longer culture experiments were attempted and in 1903 Jolly was able to maintain salamander leukocytes in vitro for a month. However despite these early experiments it is Ross Harrison who is generally regarded as the father of tissue culture. .

• Y học thường thức
• Cancer Cell
• Culture Definitions
• Thiamine HCl
• Cancer Cell Lines
• Cancer Cell Collections
• Routine Feeding
• Tạp chí nghiên cứu y học
• 3D culture and analysis
• The expression of cancer stem cell markers
• Cancer stem cell markers
• Gastric cancer cell line MKN45
• BMC Cancer
• Cancer stem cells
• Non small cell lung cancer
• Cell surface marker
• Cancer related lethality
• Cancer associated fibroblast
• Pro tumoral inflammation molecules
• Cancer progression
• Inflammation associated cell
• BMC Pulmonary Medicine
• Cell proliferation
• Esophageal squamouscell carcinomas
• Cancer cell proliferation
• Cancer stem cell
• Isolation of cancer stem like cells
• Hepatocellular carcinoma cell line HepG2
• Magnetic activated cell sorting
• Anti tumor drug resistant selection
• Primary evaluation
• Breast cancer
• Natural killer cell
• Cytotoxic T lymphocyte
• NK cell count
• Ampullary adenocarcinoma
• Cell invasion
• Ampullary cancer
• Intestinal like cell line
• Transitional cell carcinoma
• Bladder cancer
• Human cancers
• Canine bladder cancer
• Cell lines
• Cell fusion
• Pathophysiological processes
• Several physiological
• Mesothelioma stem cell
• RN5 EOS Puro2 cells
• Cancer cell repopulation
• Prostate cancer
• Cell migration
• Above mentioned cytokines
• High cancer related deaths
• Lung cancer
• Quantitative real time PCR
• Lung squamous carcinoma
• Peritoneal metastasis
• Pancreatic cancer
• 40 kDa glycoprotein
• Chimeric monoclonal IgG1
• Cervical cancer
• High aldehyde dehydrogenase
• Cell apoptosis
• Clear cell renal cell carcinoma
• In vitro models
• Primary cell culture
• Renal cancer
• Deleted in liver cancer 1
• Cancer related mortality
• Radiation therapy
• Advanced gastrointestinal cancer
• Cytokine induced killer
• Dendritic cell
• Meta analysis
• Systematic review
• All trans retinoic acid
• EGFR tyrosine kinase inhibitors
• Non small cell lung adenocarcinoma
• Cancer stem cell like cells
• Flow cytometry
• Immune checkpoint inhibitors
• Dissociated responses
• Anti programmed cell death 1
• Circulating tumor cells
• Cell free RNA
• Plasma cell free RNA
• Metastatic disease
• Major vault protein
• Local anesthetics
• Breast Cancer cells
• Cell viability
• Cell cycle
• Circulating endothelial cell
• Decreasing endothelial cell proliferation
• Objective response rate
• Insulin receptor isoform A
• Squamous cell carcinomas