TAILIEUCHUNG - Handbook of Microbiological Media, Fourth Edition part 139

Handbook of Microbiological Media, Fourth Edition part 139 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Pfennig s Medium 1 Modified for Marine Purple Sulfur Bacteria 1375 Preparation of Solution A Add components to distilled water. Mix thoroughly. Autoclave for 45 min at 15 psi pressure-121 C in 5-liter special bottle or flask with four openings at the top together with a teflon-coated magnetic bar. In this 5-liter bottle two openings for tubes are in the central silicon rubber stopper a short gas-inlet tube with a sterile cotton filter and an outlet tube for medium which reaches the bottom of the vessel at one end and has at the other end a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. The other two openings have gas-tight screw caps one of these openings is for the addition of sterile solutions and the other serves as a gas outlet. After autoclaving cool to room temperature under a N2 atmosphere with a positive pressure of atm a manometer for low pressure will be required . Saturate the cold medium with CO2 by magnetic stirring for 30 min under a CO2 atmosphere of atm. Solution B Distilled Preparation of Solution B Autoclave distilled water for 15 min at 15 psi pressure-121 C in a cotton-stoppered Erlenmeyer flask. Cool to room temperature under an atmosphere of N2 in an anaerobic jar. Solution C Composition per Vitamin Preparation of Solution C Add vitamin B12 to distilled deionized water and bring volume to . Mix thoroughly. Sparge under 100 N2 gas for 3 min. Filter sterilize. Store under N2 gas. Solution D Composition per liter Disodium ethylendiamine-tetraacetate Disodium EDTA . FeSO4-7H2O. C oC l 6H ZnCl2. Na2MoO4-2H2O. Preparation of Solution D Add components to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Solution E Composition per .

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