TAILIEUCHUNG - Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells | He et al. Journal of Biomedical Science 2011 18 39 http content 18 1 39 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells Ling-fang He1 Ting-ting Wang1 Qian-ying Gao1 Guang-feng Zhao1 Ya-hong Huang1 Li-ke Yu2 and Ya-yi Hou1 Abstract Background Stanniocalcin-1 STC-1 is up-regulated in several cancers including gastric cancer. Evidences suggest that STC-1 is associated with carcinogenesis and angiogenic process. However it is unclear on the exact role for STC-1 in inducing angiogenesis and tumorigeneisis. Method BGC STC cells high-expression of STC-1 and BGC shSTC cells low- expression of STC-1 were constructed to investigate the effect of STC-1 on the xenograft tumor growth and angiogenesis in vitro and in vivo. ELISA assay was used to detect the expression of vascular endothelial growth factor VEGF in the supernatants. Neutralizing antibody was used to inhibit VEGF expression in supernatants. The expression of phosphorylated -PKCpII phosphorylated -ERK1 2 and phosphorylated -P38 in the BGC treated with STC-1protein was detected by western blot. Results STC-1 could promote angiogenesis in vitro and in vivo and the angiogenesis was consistent with VEGF expression in vitro. Inhibition of VEGF expression in supernatants with neutralizing antibody markedly abolished angiogenesis induced by STC-1 in vitro. The process of STC-1-regulated VEGF expression was mediated via PKCpII and ERK1 2. Conclusions STC-1 promotes the expression of VEGF depended on the activation of PKCpil and ERK1 2 pathways. VEGF subsequently enhances tumor angiogenesis which in turn promotes the gastric tumor growth. Keywords STC-1 angiogenesis VEGF PKCPII ERK1 2 Background Development of gastric cancer involves multiple factor changes that lead to the transformation of human .

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