TAILIEUCHUNG - s Membrane proteomic analysis of pancreatic cancer cells

Background: Pancreatic cancer is one of the most aggressive human tumors due to its high potential of local invasion and metastasis. The aim of this study was to characterize the membrane proteomes of pancreatic ductal adenocarcinoma (PDAC) cells of primary and metastatic origins, and to identify potential target proteins related to metastasis of pancreatic cancer. Methods: Membrane/membrane-associated proteins were isolated from AsPC-1 and BxPC-3 cells and identified with a proteomic approach based on SDS-PAGE, in-gel tryptic digestion and liquid chromatography with tandem mass spectrometry (LC-MS/MS). X! Tandem was used for database searching against the SwissProt human protein database. Results: We. | Liu et al. Journal of Biomedical Science 2010 17 74 http content 17 1 74 BIN sc The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Membrane proteomic analysis of pancreatic cancer cells Xiaojun Liu1 Min Zhang1 Vay Liang W Go2 Shen Hu1 3 Abstract Background Pancreatic cancer is one of the most aggressive human tumors due to its high potential of local invasion and metastasis. The aim of this study was to characterize the membrane proteomes of pancreatic ductal adenocarcinoma PDAC cells of primary and metastatic origins and to identify potential target proteins related to metastasis of pancreatic cancer. Methods Membrane membrane-associated proteins were isolated from AsPC-1 and BxPC-3 cells and identified with a proteomic approach based on SDS-PAGE in-gel tryptic digestion and liquid chromatography with tandem mass spectrometry LC-MS MS . X Tandem was used for database searching against the SwissProt human protein database. Results We identified 221 208 proteins from AsPC-1 and BxPC-3 cells respectively most of which are membrane or membrane-associated proteins. A hundred and nine proteins were found in both cell lines while the others were present in either AsPC-1 or BxPC-3 cells. Differentially expressed proteins between two cell lines include modulators of cell adhesion cell motility or tumor invasion as well as metabolic enzymes involved in glycolysis tricarboxylic acid cycle or nucleotide lipid metabolism. Conclusion Membrane proteomes of AsPC-1 metastatic and BxPC-3 primary cells are remarkably different. The differentially expressed membrane proteins may serve as potential targets for diagnostic and therapeutic interventions. Introduction Pancreatic cancer is one of the most aggressive human malignancies. Despite the advances in therapeutic strategies including surgical techniques as well as local and systemic adjuvant therapies the .

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