TAILIEUCHUNG - Molecular oncology focus - Is carcinogenesis a 'mitochondriopathy'?

Abstract Mitochondria are sub-cellular organelles that produce adenosine triphosphate (ATP) through oxidative phosphorylation (OXPHOS). As suggested over 70 years ago by Otto Warburg and recently confirmed with molecular techniques, alterations in respiratory activity and in mitochondrial DNA (mtDNA) appear to be common features of malignant cells. Somatic mtDNA mutations have been reported in many types of cancer cells, and some reports document the prevalence of inherited mitochondrial DNA polymorphisms in cancer patients. Nevertheless, a careful reanalysis of methodological criteria and methodology applied in those reports has shown that numerous papers can't be used as. | Czarnecka et al. Journal of Biomedical Science 2010 17 31 http content 17 1 31 a NSC Tha cost of publication In Journal of Blomodlcal Science Is boms by tlM National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE REVIEW Open Access Molecular oncology focus - Is carcinogenesis a mitochondriopathy Anna M Czarnecka 1 Jerzy S Czarnecki2 Wojciech Kukwa3 Francesco Cappello4 Anna Scinska3 and Andrzej Kukwa3 Abstract Mitochondria are sub-cellular organelles that produce adenosine triphosphate ATP through oxidative phosphorylation OXPHOS . As suggested over 70 years ago by Otto Warburg and recently confirmed with molecular techniques alterations in respiratory activity and in mitochondrial DNA mtDNA appear to be common features of malignant cells. Somatic mtDNA mutations have been reported in many types of cancer cells and some reports document the prevalence of inherited mitochondrial DNA polymorphisms in cancer patients. Nevertheless a careful reanalysis of methodological criteria and methodology applied in those reports has shown that numerous papers can t be used as relevant sources of data for systematic review meta-analysis or finally for establishment of clinically applicable markers. In this review technical and conceptual errors commonly occurring in the literature are summarized. In the first place we discuss why many of the published papers cannot be used as a valid and clinically useful sources of evidence in the biomedical and healthcare contexts. The reasons for introduction of noise in data and in consequence - bias for the interpretation of the role of mitochondrial DNA in the complex process of tumorigenesis are listed. In the second part of the text practical aspects of mtDNA research and requirements necessary to fulfill in order to use mtDNA analysis in clinics are shown. Stringent methodological criteria of a case-controlled experiment in molecular medicine are indicated. In the third part we suggest what lessons can be learned

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