TAILIEUCHUNG - Biosensors for Health Environment and Biosecurity Part 7

Tham khảo tài liệu 'biosensors for health environment and biosecurity part 7', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 202 Biosensors for Health Environment and Biosecurity steps since the access of marker species to the electrode surface became more difficult and the semicircle overlapped the straight line which may disappear depending on how the electrode surface has been blocked. The values of EEC elements obtained in the simulation must be compared with those previously reported for the same or similar systems Ferreira et al. 2009 . In some cases the stepwise process of the immunosensor construction was studied by EIS Yuan et al. 2009 and the real impedance measured in Fe CN 63- 4- redox couple PBS solution pH was higher for the bare glassy carbon electrode than for the electrode modified with gold nanoparticles due to the increase in the active area of the electrode. In the next step the electrode was modified with nickel hexacianoferrate the charge transfer resistance increased due to the partial blocking of the electrode surface. However the RCT value decreased again when gold nanoparticles were incorporated to this modified electrode. The decrease of RCT can be related to the increase of the conductivity of the system. When more modifications with organic molecules were performed the RCT increased as expected. Recently more detailed studies on the surface modification using EIS with Ferreira et al. 2009 and without Ferreira et al. 2010 a redox marker Fe CN 6 4- in the solution were performed. In the first study diffusion coefficients of the marker RCT and Cdl values were obtained and compared with data of literature for the bare gold-based SPE. The values of apparent RCT and surface coverage of SPE with CYS CYS-GA and CYS-GA-Tc85 protein were determined based on a treatment of impedance previously developed for Q values lower Gueshi et al. 1978 Matsuda et al. 1979 and higher Finklea et al. 1993 than . The modified electrode was interpreted as a perforated layer with the transfer reaction occurring at the uncovered regions of the electrode surface which represent .

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