TAILIEUCHUNG - Báo cáo khoa học: Involvement of mitochondrial permeability transition pore opening in a-bisabolol induced apoptosis

a-Bisabolol is a natural monocyclic sesquiterpene alcohol. It has been used in cosmetics for hundreds of years because of its perceived skin-healing properties. a-Bisabolol is known to have anti-irritant, anti-inflammatory and antimicrobial properties. In precedent studies, we described how a-bisabolol exerts a selective pro-apoptotic action towards transformed cells [Cavalieri E et al. | ễFEBS Journal Involvement of mitochondrial permeability transition pore opening in a-bisabolol induced apoptosis Elisabetta Cavalieri1 z Christian Bergamini2 z Sofia Mariotto1 Serena Leoni2 Luigi Perbellini3 Elena Darra1 Hisanori Suzuki1 Romana Fato2 and Giorgio Lenaz2 1 Dipartimento di Scienze Morfologico-Biomediche Universita di Verona Italy 2 Dipartimento di Biochimica G. Moruzzi Universita di Bologna Italy 3 Dipartimento di Medicina e Sanita Pubblica Universita di Verona Italy Keywords a-bisabolol apoptosis cyclosporine A mitochondria mitochondrialpermeability transition pore Correspondence H. Suzuki Dipartimento di Scienze Morfologico-Biomediche University di Verona Strada Le Grazie 8 37134 Verona Italy Fax 39 045 802 7170 Tel 39 045 802 7167 E-mail These authors contributed equally to this work Received 16 April2009 revised 15 May 2009 accepted 21 May 2009 doi a-Bisabolol is a natural monocyclic sesquiterpene alcohol. It has been used in cosmetics for hundreds of years because of its perceived skin-healing properties. a-Bisabolol is known to have anti-irritant anti-inflammatory and antimicrobial properties. In precedent studies we described how a-bisabolol exerts a selective pro-apoptotic action towards transformed cells Cavalieri E et al. 2004 Biochem Biophys Res Commun 315 589-594 and its uptake is mediated by lipid rafts on the plasma membrane Darra E et al. 2008 Arch Biochem Biophys 476 113-123 . In this study we hypothesize that the intracellular target of a-bisabolol may be the mitochondrial permeability transition pore mPTP . To evaluate this hypothesis we used one transformed cell line human glioma T67 in comparison with a nontransformed one human fibroblasts . We assessed the effect of a specific mPTP inhibitor cyclosporine A on the toxic action of a-bisabolol. Results show that the a-bisabolol-induced decrease in oxygen consumption is abolished by the addition of cyclosporine A in T67 cells .

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