TAILIEUCHUNG - Báo cáo khoa học: Identification of structural determinants for inhibition strength and specificity of wheat xylanase inhibitors TAXI-IA and TAXI-IIA

Triticum aestivum xylanase inhibitor (TAXI)-type inhibitors are active against microbial xylanases from glycoside hydrolase family 11, but the inhibition strength and the specificity towards different xylanases differ between TAXI isoforms. Mutational and biochemical analyses of TAXI-I, TAXI-IIA andBacillus subtilisxylanase A showed that inhibition strength and specificity depend on the identity of only a few key residues of inhibitor and xylanase [Fierens K et al. (2005) FEBS J272, 5872–5882; Raedschelders Get al | Identification of structural determinants for inhibition strength and specificity of wheat xylanase inhibitors TAXI-IA and TAXI-IIA Annick Pollet1 Stefaan Sansen2 Gert Raedschelders3 Kurt Gebruers1 Anja Rabijns2 Jan A. Delcour1 and Christophe M. Courtin1 1 Laboratory of Food Chemistry and Biochemistry and Leuven Food Science and Nutrition Research Centre LFoRCe Katholieke Universiteit Leuven Belgium 2 Laboratory for Biocrystallography Katholieke Universiteit Leuven Belgium 3 Laboratory of Gene Technology Katholieke Universiteit Leuven Belgium Keywords Bacillus subtilis inhibition Triticum aestivum X-ray structure xylanase Correspondence C. M. Courtin Laboratory of Food Chemistry and Biochemistry and Leuven Food Science and Nutrition Research Centre LFoRCe Katholieke Universiteit Leuven Kasteelpark Arenberg 20 - bus 2463 B-3001 Leuven Belgium Fax 32 16 321997 Tel 32 16 321917 E-mail Note The atomic coordinates and structure factors of BSX TAXI-IA PDB code 2B42 and BSX-rTAXI-IIA PDB code 3HD8 are deposited in the Protein Data Bank Research Collaboratory for Structural Bioinformatics Rutgers University New Brunswick NJ USA http Received 12 March 2009 revised 15 May 2009 accepted 20 May 2009 doi Triticum aestivum xylanase inhibitor TAXI -type inhibitors are active against microbial xylanases from glycoside hydrolase family 11 but the inhibition strength and the specificity towards different xylanases differ between TAXI isoforms. Mutational and biochemical analyses of TAXI-I TAXI-IIA and Bacillus subtilis xylanase A showed that inhibition strength and specificity depend on the identity of only a few key residues of inhibitor and xylanase Fierens K et al. 2005 FEBS J 272 5872-5882 Raedschelders G et al. 2005 Biochem Biophys Res Commun 335 512-522 Sorensen JF Sibbesen O 2006 Protein Eng Des Sel 19 205-210 Bourgois TM et al. 2007 J Biotechnol 130 95-105 . Crystallographic analysis of the .

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