TAILIEUCHUNG - Salmonella A Dangerous Foodborne Pathogen Part 18

Tham khảo tài liệu 'salmonella a dangerous foodborne pathogen part 18', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 414 Salmonella - A Dangerous Foodborne Pathogen Present commercial detection system for Salmonella spp. can be classified into four categories. The first traditional method which uses culture medium and observe colony morphology formed on it. This requires at least four days and experienced skill to perform biological tests but it is the only common method authorized throughout the world for now. The second Enzyme-Linked Immuno-Sorbent Assay ELISA detects certain bacteria using immune reaction between antibody and antigen specific for them. This method is easy to use because it makes color change or forms lines but it can be applied only for those which has specific toxin protein and requires more than 106 CFU ml for detection which needs 16 hours of incubation. The third Adenosine triphosphate ATP detection kit detects level of bacterial contamination by the amount of ATP in sample. This method can not be used for identification of bacteria because it can only tell including the total amount of ATP from food. This is usually used for comparing hygiene level before and after washing. The fourth genetic method which is based on PCR is highly specific and sensitive enough to detect 100 CFU ml of bacteria but at the same time it can detect even the dead cells after processing or cooking food because of the high sensitivity. Advanced PCR technologies Multiplex PCR Multiplex PCR can amplify two or more amplicons in a single PCR reaction. For multiplex PCR each primer set is designed to amplify its target gene and make a PCR product of certain size to the target gene. To perform a multiplex PCR the concentration of primers Mg2 free dNTPs and polymerase must be optimized to allow synthesis of the genes of interest And also the PCR reaction temperature parameters must be optimized to the best average for amplicon production for all primer sets. This technique saves time and labor more than one target DNA sequence can be detected in each reaction It might not be .

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