TAILIEUCHUNG - Báo cáo lâm nghiệp: "Use of chloroplast microsatellites to differentiate oak populations"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Use of chloroplast microsatellites to differentiate oak populations. | Ann. For. Sci. 61 2004 825-830 INRA EDP Sciences 2005 DOI forest 2004078 825 Original article Use of chloroplast microsatellites to differentiate oak populations Marie-France DEGUILLOUXa b Marie-Hélène PEMONGEa Rémy J. PETITa a Institut National de la Recherche Agronomique Station de Recherches Forestieres 69 route d Arcachon 33612 Cestas Cedex France b Centre Technique du Bois et de l Ameublement 10 avenue de St-Mandé 75012 Paris France Received 13 March 2003 accepted 22 January 2004 Abstract - The possibility to use chloroplast microsatellites cpSSRs instead of restriction analysis of PCR-amplified DNA fragments to differentiate oak populations was tested in two economically important tree species Quercuspetraea and Quercus robur. The level and pattern of inter- and intraspecific cpDNA variation were studied over 48 French populations using a total of 24 cpSSR loci. The same pattern of low intrapopulation diversity and high population differentiation was noted with both types of markers since there was an almost total redundancy of haplotypes identified with both techniques. Overall our results indicate that chloroplast microsatellites can be used for haplotype discrimination in many contexts including certification or traceability of oak material. microsatellites cpSSR PCR-RFLP haplotypic diversity population genetics Résumé - Utilisation des microsatellites chloroplastiques pour differencier des populations de chênes. Nous avons cherché à comparer l efficacité de deux types de marqueurs chloroplastiques les marqueurs microsatellites cpSSR et marqueurs PCR-RFLP cpRFLP à différencier des populations de chênes sessiles et pédonculés deux espèces d arbres forestiers économiquement importantes. Le niveau et la structuration de la diversité inter et intra-spécifique ont été mesurés au sein de 48 populations íranẹaises au travers de l analyse de 24 microsatellites chloroplastiques. Une faible diversité intra-population ainsi qu une forte différenciation des .

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