TAILIEUCHUNG - Báo cáo y học: " NF- B subunits RELB C-Rel RELA p50 p52 bound DNA b EMS"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: NF- B subunits RELB C-Rel RELA p50 p52 bound DNA b EMSA. | Genome Biology NF-kB subunits o A o 0 relb C-Rel RELA p50 p52 Protein-DNA Binding microarrays Electrophoretic Mobility Shift Assay EMSA UV-laser footprinting of TF-bound DNA sequences Create TF-binding profiles for dimers Extensive characterization of NF-kB binding uncovers non-canonical motifs and advances the interpretation of genetic functional traits Wong et al. Wong et al. Genome Biology 2011 12 R70 http 2011 12 7 R70 29 July 2011 2 BioMed Central Wong et al. Genome Biology 2011 12 R70 http 2011 12 7 R70 Genome Biology RESEARCH Open Access Extensive characterization of NF-kB binding uncovers non-eanonieal motifs and advances the interpretation of genetic functional traits 1 f A T- 1 f 1 f 1 2 3 Daniel Wong Ana Teixeira Spyros Oikonomopoulos Peter Humburg Imtiaz Nisar Lone David Saliba Trevor Siggers4 Martha Bulyk4 5 6 Dimitar Angelov2 Stefan Dimitrov7 Irina A Udalova3 and Jiannis Ragoussis 1 Abstract Background Genetic studies have provided ample evidence of the influence of non-coding DNA polymorphisms on trait variance particularly those occurring within transcription factor binding sites. Protein binding microarrays and other platforms that can map these sites with great precision have enhanced our understanding of how a single nucleotide polymorphism can alter binding potential within an in vitro setting allowing for greater predictive capability of its effect on a transcription factor binding site. Results We have used protein binding microarrays and electrophoretic mobility shift assay-sequencing EMSA-Seq a deep sequencing based method we developed to analyze nine distinct human NF-kB dimers. This family of transcription factors is one of the most extensively studied but our understanding of its DNA binding preferences has been limited to the originally described consensus motif GGRRNNYYCC. We highlight differences between NF-kB family members and also put under the spotlight non-canonical motifs that have so far .

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