TAILIEUCHUNG - báo cáo khoa học: "Expression of a quantitative character radius incompletus, temperature effects, and localization of a mobile genetic element Dm-412 in Drosophila melanogaster"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Expression of a quantitative character radius incompletus, temperature effects, and localization of a mobile genetic element Dm-412 in Drosophila melanogaster | Génét. Sél. Evol. 1988 20 2 159-180 Expression of a quantitative character radius incompletus temperature effects and localization of a mobile genetic element Dm-412 in Drosophila melanogaster . VASILYEVA . ZABANOV . RATNER . ZHIMULEV . PROTOPOPOV and . BELYAEVA Institute of Cytology and Genetics Academy of Sciences of the USSR Siberian Branch Novosibirsk 630090 USSR Summary The radius incompletus mutation ri causes a gap in the radial wing vein L2. A control line riC of the mutation ri was selected for increase and decrease of radial vein. In the riSN strain extreme expression of this quantitative character is almost complete elimination of L2 negative selection SN . In the riSP strain the L2 vein is almost restored positive selection SP . Similar changes in the expression of ri were obtained by altering the temperature at which flies were cultured during the pupal stage. Surprisingly a single temperature change treatment gave rise to strains in which the modified expression of ri was stably inherited through more than 150 generations. There are two temperature sensitive phases within the pupal stage one at 113 5 h and the other at 149 5 h after egg laying. The line derived from temperature change at 113 5 h riTU3 had an L2 length two times less than in the original line riC. The line derived from temperature change at 149 5 h riTI49 had an L2 length times greater than in riC. Genetic analysis demonstrated that expression of the ri phenotype was affected by polygenic modifiers. All five lines were investigated by in situ hybridisation of DNA clone of MGE mobile genetic element Dm-412 to larval polytene chromosomes. A tree of similarity of MGE hybridization patterns was built by the methods of matrix clustering. The lines with the most similar expressions of the ri phenotype and riT113 riSP and riT149 correspondingly were found to have the most similar patterns of Dm-412 localization and transpositions. The Dm-412 transpositions in 5-and

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