TAILIEUCHUNG - Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione

A novel metallobridged bis(b-cyclodextrin)s 2[bis(b-CD)s 2] was synthesized and characterized by means of 1 H NMR, IR, element analysis and redox iodometric titration. The fluorescence of metallobridged bis(b-CD)s 2 was weak compared with bis(b-CD)s 1because of the paramagnetism of copper (II) ions. | ễFEBS Journal A novel metallobridged bis b-cyclodextrin s fluorescent probe for the determination of glutathione Bo Tang Fang Liu Kehua Xu and Lili Tong College of Chemistry ChemicalEngineering and Materials Science Shandong Normal university Jinan China Keywords competitive complexation glutathione metallobridged bis b-cyclodextrin s molecular recognition spectrofluorimetry Correspondence B. Tang College of Chemistry Chemical Engineering and Materials Science Engineering Research Center of Pesticide and Medicine Intermediate Clean Production Ministry of Education Key Laboratory of Molecular and Nano Probes Ministry of Education Shandong Normal University Jinan 250014 China Fax 86 531 8618 0017 Tel 86 531 8618 0010 E-mail tangb@ A novel metallobridged bis b-cyclodextrin s 2 bis b-CD s 2 was synthesized and characterized by means of 1H NMR IR element analysis and redox iodometric titration. The fluorescence of metallobridged bis b-CD s 2 was weak compared with bis b-CD s 1 because of the paramagnetism of copper II ions. Glutathione was able to form complexes with copper II derived from the metallobridged bis b-CD s 2. This competitive complexa-tion with copper II may lead to a significant fluorescence recovery of the bis b-CD s. Therefore a rapid and simple spectrofluorimetric method was developed for the determination of glutathione. The analytical application for glutathione was investigated in NaCh Pi pH at room temperature. The linear range of the method was Limol L 1 with a detection limit of nmol L-1. There was no interference from the plasma constituents. The proposed method had been successfully used to determine glutathione in human plasma. Received 30 November 2007 revised 13 January 2008 accepted 25 January 2008 doi Reduced glutathione GSH y-Glu-Cys-Gly a principal non-protein thiol compound plays an important role in many biological processes such as transport protein synthesis catabolism .

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